Krízová P, Kalmusová J, Musílek M
Národní referencní laborator pro meningokokové nákazy, Státní zdravotní ustav, Praha.
Epidemiol Mikrobiol Imunol. 2006 Nov;55(4):140-50.
The study objective is to present results of clonal analysis of Neisseria meningitidis isolates from invasive meningococcal disease in the Czech Republic in 1993-2005. The method of multilocus sequence typing (MLST) revealed genetic heterogeneity of serogroup B isolates and homogeneity of serogroup C isolates. Three clonal sequence type complexes (STC-18, STC-32 and STC-41/44) prevailed among serogroup B isolates and almost a quarter of isolates have ST unclassifiable into clonal complexes. STC-11 showed clear predominance among serogroup C isolates. Hypervirulent clonal complex STC-11 prevailed in 1993-2004 while another clonal complex, STC-41/44, became more widespread for the first time in 2005. MLST analysis showed differences between the meningococcal population in the Czech Republic and those in western European countries and elsewhere. The results of clonal analysis of Neisseria meningitidis isolates from invasive meningococcal disease provide background information for evaluation of the adequacy of recommended measures in the focus of the disease and for updating the recommended vaccination strategy in the Czech Republic.
本研究的目的是呈现1993 - 2005年捷克共和国侵袭性脑膜炎球菌病中分离出的脑膜炎奈瑟菌的克隆分析结果。多位点序列分型（MLST）方法揭示了B群分离株的遗传异质性和C群分离株的同质性。在B群分离株中，三种克隆序列类型复合体（STC - 18、STC - 32和STC - 41/44）占主导地位，近四分之一的分离株其序列类型无法归类到克隆复合体中。STC - 11在C群分离株中表现出明显的优势。高毒力克隆复合体STC - 11在1993 - 2004年占主导地位，而另一个克隆复合体STC - 41/44在2005年首次变得更为广泛。MLST分析显示捷克共和国的脑膜炎球菌群体与西欧国家及其他地区的存在差异。侵袭性脑膜炎球菌病中分离出的脑膜炎奈瑟菌的克隆分析结果为评估疾病重点防控措施的充分性以及更新捷克共和国推荐的疫苗接种策略提供了背景信息。
