Pliquett Uwe, Weaver James C
Institut für Bioprozess-und Analysenmesstechnik e.V., Heilbad Heiligenstadt 37308, Germany.
IEEE Trans Biomed Eng. 2007 Mar;54(3):536-8. doi: 10.1109/TBME.2006.886828.
Electrical creation of aqueous pathways across the skin's outer layer [stratum corneum (SC)] provides an approach to transdermal delivery of medium-size water-soluble compounds. However, nerve stimulation should be avoided. Here, we show that a microstructured electrode array can significantly confine the electric field to the nerve-free SC. The prototype electrode-reservoir device (ERD) contains field-confining electrodes and a fluorescent drug surrogate [sulphorhodamine (SR)]. In vivo human experiments at the forearm with approximately rectangular voltage pulses up to 500 V and 1-ms duration cause electroporation as measured by skin resistance change but only rarely caused sensation. Human skin in vitro experiments with such pulses up to 300 V transported SR across the SC. Our results are supported by a model's prediction of the field in the ERD and nearby tissue.
通过电刺激在皮肤外层[角质层（SC）]中创建水性通道，为中等大小的水溶性化合物的透皮递送提供了一种方法。然而，应避免神经刺激。在此，我们表明，微结构化电极阵列可将电场显著限制在无神经的角质层中。原型电极-储液器装置（ERD）包含电场限制电极和荧光药物替代物[磺基罗丹明（SR）]。在前臂进行的人体体内实验中，使用高达500 V、持续时间为1 ms的近似矩形电压脉冲，通过皮肤电阻变化测量可知会引起电穿孔，但很少引起感觉。使用高达300 V的此类脉冲进行的人体皮肤体外实验使SR穿过了角质层。我们的结果得到了ERD及附近组织中电场模型预测的支持。
