Lee Shun, McAuliffe Daniel J, Kollias Nikiforos, Flotte Thomas J, Doukas Apostolos G
Department of Dermatology, Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Lasers Surg Med. 2002;31(3):207-10. doi: 10.1002/lsm.10099.
Photomechanical waves (PWs) render the stratum corneum permeable and allow molecules to diffuse into the epidermis. The aim of this study was to investigate the probe size that could be delivered through the stratum corneum and into the epidermis.
STUDY DESIGN/MATERIALS AND METHODS: A single PW was applied onto the rat skin in vivo. Aqueous suspensions of fluorescent microspheres, 100 nm in diameter, were used as probes for transdermal delivery. The presence of the microspheres in the epidermis was measured by a fiber-based spectrofluorimeter after the stratum corneum was removed by tape-stripping (TS).
Exposure of the rat skin to a PW permeabilized the stratum corneum and allowed the fluorescent microspheres to diffuse into the epidermis.
The experiments show that PWs can facilitate the delivery of very large molecules and probes into the epidermis.
光机械波（PW）可使角质层具有通透性，从而使分子扩散进入表皮。本研究旨在探究能够透过角质层并进入表皮的探针大小。
研究设计/材料与方法：在大鼠活体皮肤上施加单个PW。使用直径为100纳米的荧光微球水悬浮液作为透皮给药的探针。通过胶带剥离（TS）去除角质层后，用基于光纤的荧光光谱仪测量表皮中微球的存在情况。
大鼠皮肤暴露于PW后，角质层通透性增加，荧光微球能够扩散进入表皮。
实验表明，PW可促进非常大的分子和探针进入表皮。
