Cole Lynette K, Luu Dao H, Rajala-Schultz Paivi J, Meadows Cheyney, Torres Audrey H
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
Vet Dermatol. 2007 Apr;18(2):115-9. doi: 10.1111/j.1365-3164.2007.00583.x.
The purpose of this study was to evaluate the in vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole in purified water on Malassezia organisms from dogs with otitis externa. Malassezia organisms were collected from ear swab samples from the external ear canal of 19 dogs with otitis externa plus one control strain of Malassezia pachydermatis. Three test solutions were evaluated: ER (EDTA, tromethamine, benzyl alcohol), ER + keto (EDTA, tromethamine, benzyl alcohol, ketoconazole), and H2O (purified water). Ten-millilitre aliquots of each test solution was transferred into 20 tubes and inoculated with one of the isolates (1 tube per isolate: 19 clinical and 1 control strain). Samples were retrieved from each tube at five time points (0, 15, 30, 45 and 60 min), transferred to Petri dishes, mixed with Sabouraud dextrose agar supplemented with 0.5% Tween 80 and incubated. Following incubation, the plates were examined for growth and colonies counted as colony-forming units per millilitre. The data were analysed using a repeated measures analysis, with pair-wise comparisons of solution-time combinations. There was a significant reduction in Malassezia growth in ER + keto at all time points (P < 0.0001) compared to time zero. Neither ER nor H2O had any effect on the growth of Malassezia. ER + keto was significantly more effective in reducing Malassezia growth (P < 0.0001) at all time points compared to both ER and H2O. ER + keto may be useful in the treatment of Malassezia otitis externa. Future studies should be performed to evaluate the in vivo efficacy of ER + keto as treatment for otic infections caused by Malassezia.
本研究的目的是评估一种含有 tromethamine、EDTA、苯甲醇和 0.1%酮康唑的耳冲洗液在纯水中对来自患有外耳炎的犬的马拉色菌属微生物的体外活性。从 19 只患有外耳炎的犬的外耳道耳拭子样本以及一株厚皮马拉色菌对照菌株中收集马拉色菌属微生物。评估了三种测试溶液：ER（EDTA、tromethamine、苯甲醇）、ER + 酮康唑（EDTA、tromethamine、苯甲醇、酮康唑）和 H₂O（纯净水）。将每种测试溶液的 10 毫升等分试样转移到 20 个试管中，并用其中一种分离株接种（每个分离株 1 管：19 个临床菌株和 1 个对照菌株）。在五个时间点（0、15、30、45 和 60 分钟）从每个试管中取出样本，转移到培养皿中，与补充有 0.5%吐温 80 的沙氏葡萄糖琼脂混合并孵育。孵育后，检查平板上的生长情况，并将菌落计数为每毫升菌落形成单位。使用重复测量分析对数据进行分析，并对溶液 - 时间组合进行成对比较。与零时间相比，ER + 酮康唑在所有时间点的马拉色菌生长均显著降低（P < 0.0001）。ER 和 H₂O 对马拉色菌的生长均无任何影响。与 ER 和 H₂O 相比，ER + 酮康唑在所有时间点显著更有效地降低马拉色菌生长（P < 0.0001）。ER + 酮康唑可能有助于治疗马拉色菌性外耳炎。未来应进行研究以评估 ER + 酮康唑作为马拉色菌引起的耳部感染治疗方法的体内疗效。
