Ye Fanglei, Guo Xiangdong, Lou Weihua, Xie Tianfei
Department of Otorhinolaryngology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou,450052, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Dec;20(24):1135-7.
To investigate the protective effects of minocycline against apoptosis of olfactory sensory neurons induced by sinusitis.
Sixty adult SD rats were divided randomly into three groups: control group (group A), sinusitis group (group B) and intravenous injection of minocycline group (group C). The rats were sacrificed at 1,3,5 d and 7 d after surgery, and then the tissue sections of olfactory epithelium were stained and evaluated with hematoxylin and eosin (HE) and immunohistochemistry method.
The histopathological changes in maxillary sinus epithelium tissues observed with he staining microscopic examination in group B and C were worse than that in group A. Significant difference of the thickness of olfactory epithelium and Caspase-3 activity was demonstrated in group B and group C.
The result demonstrated that minocycline could inhibit expression of Caspase-3 in rats with sinusitis.
探讨米诺环素对鼻窦炎诱导的嗅觉感觉神经元凋亡的保护作用。
将60只成年SD大鼠随机分为三组：对照组（A组）、鼻窦炎组（B组）和静脉注射米诺环素组（C组）。术后1、3、5和7天处死大鼠，然后用苏木精-伊红（HE）染色和免疫组化方法对嗅上皮组织切片进行染色和评估。
B组和C组经HE染色显微镜检查观察到的上颌窦上皮组织病理变化比A组更严重。B组和C组在嗅上皮厚度和Caspase-3活性方面存在显著差异。
结果表明米诺环素可抑制鼻窦炎大鼠Caspase-3的表达。
