Zhang Xianghong, Zhao Lin, Sun Bin, Liang Jianmin, Xu Min, Hao Hong, Wang Botao
Department of Otolaryngology, Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Sep;22(17):789-93.
To study the ultrastructure change and cell apoptosis in the olfactory mucosa of rats suffering from chronic sinusitis, and aim to elucidate the possible mechanism and provide a novel concept of therapy approach to olfactory disease caused by chronic sinusitis.
Light microscope and transmission electron microscope were used to observe the ultrastructure changes in the olfactory mucosa of 20 rats suffering from chronic sinusitis and that in 20 cases of normal olfactory mucosa. By the method of TUNEL (TdT-mediated dUTP nick end labeling), we studied the apoptosis index (AI) in the olfactory mucosa of 20 rats suffering from chronic sinusitis and that in 20 cases of normal olfactory mucosa.
(1) Observation by transmission electron microscope: In the olfactory epithelium of the rats group suffering from chronic sinusitis, the ultrastructure of the olfactory epithelium showed some abnormal changes: Olfactory epithelium cells showed a variety of degeneration ornecrosis, the olfactory epithelium were quite disorganized. Supporting cell nuclei were oval, the nuclei of the cell aggregated as the plaque and fringe collection, the density of cytoplasm decreased. Surface microvillus of the olfactory cells reduced and disappeared, part of the karyolemma defected like morphology change about cell apoptosis. Inflammatory cells infiltrated. Basement membrane was exposed and cells were quite disorganized. Olfactory gland cells were prismatic, cell spaces were widen and secretion increased in the cavosurface. In the olfactory mucosa of normal group, the ultrastructure of olfactory epithelium showed some normal changes: Olfactory epithelium were quite regularity, supporting cells and olfactory cells were radial arrangement, basement membrane were integrated, the structure of olfactory gland were completed, microvirus were in richet in cavosurface and slight of serositim were in the cavosurface; (2) The apoptosis index (AI) in the olfactory epithelium of the rats group suffering from chronic sinusitis was higher than that in normal group (P < 0.05).
There is a ultrastructural change from the surface to the inner of olfactory mucosa in rats suffering from chronic sinusitis. There is notable apoptosis in the olfactory epithelium and propria lamina of rats suffering from chronic sinusitis.
研究慢性鼻窦炎大鼠嗅黏膜的超微结构变化及细胞凋亡情况，旨在阐明其可能机制，为慢性鼻窦炎所致嗅觉障碍的治疗提供新的思路。
采用光镜和透射电镜观察20只慢性鼻窦炎大鼠及20例正常嗅黏膜的超微结构变化；运用TUNEL（TdT介导的dUTP缺口末端标记法）检测20只慢性鼻窦炎大鼠及20例正常嗅黏膜的凋亡指数（AI）。
（1）透射电镜观察：慢性鼻窦炎大鼠组嗅上皮超微结构出现异常改变：嗅上皮细胞呈现多种变性或坏死，排列紊乱；支持细胞核呈椭圆形，核仁聚集呈斑块状和边缘状，细胞质密度降低；嗅细胞表面微绒毛减少、消失，部分核膜出现类似细胞凋亡的形态改变；有炎性细胞浸润；基底膜暴露，细胞排列紊乱。嗅腺细胞呈柱状，细胞间隙增宽，腔面分泌增多。正常组嗅黏膜嗅上皮超微结构正常：嗅上皮排列规则，支持细胞和嗅细胞呈放射状排列，基底膜完整，嗅腺结构完整，腔面微绒毛丰富，有少量浆液性物质；（2）慢性鼻窦炎大鼠组嗅上皮凋亡指数（AI）高于正常组（P<0.05）。
慢性鼻窦炎大鼠嗅黏膜从表面至深层存在超微结构改变，其嗅上皮及固有层存在明显的细胞凋亡。
