Wu Chi-Ying, Lee Wei-Hwa, Wang Jia-Yi, Chiang Hung, Chang Junn-Liang, Tsai Wen-Chiuan, Sheu Lai-Fa, Jin Jong-Shiaw
Department of Pathology, Tri-Service General Hospital, Taipei , Taiwan 114, ROC.
Chin J Physiol. 2006 Dec 31;49(6):298-304.
Accumulated evidence reveals that increased cyclooxygenase-2 (COX-2) is involved in the development of colorectal cancer. Our purpose was to quantitate COX-2 expression in colorectal cancers using tissue microarray analysis and look for an association with clinicopathological stage. Immunohistochemical analysis of COX-2 was performed in tissue microarray slides containing 90 specimens including 32 well-differentiated, 35 moderately differentiated, and 23 poorly differentiated colorectal adenocarcinomas. All colorectal adenocarcinomas showed significant immunohistochemical expression of COX-2 when compared to normal colon epithelia. However, there was no significant difference in immunostaining scores between poorly, moderately, and well-differentiated tumors (195 +/- 28, 214 +/- 26 and 200 +/- 24, respectively). The COX-2 immunostaining score correlated significantly with T stage (P < 0.05) but not with N or M stage. The positive expression rates of CK20 were 97% for well-differentiated, 94% for moderately differentiated, and 65% for poorly differentiated colorectal adenocarcinomas, suggesting that CK20 may not be an effective discriminator between poorly differentiated colorectal adenocarcinoma and metastatic adenocarcinoma.
越来越多的证据表明，环氧化酶-2（COX-2）表达增加与结直肠癌的发生发展有关。我们的目的是通过组织芯片分析定量检测结直肠癌中COX-2的表达，并寻找其与临床病理分期的相关性。对包含90个标本的组织芯片载玻片进行COX-2免疫组织化学分析，其中包括32例高分化、35例中分化和23例低分化结直肠腺癌。与正常结肠上皮相比，所有结直肠腺癌均显示出显著的COX-2免疫组织化学表达。然而，低分化、中分化和高分化肿瘤之间的免疫染色评分无显著差异（分别为195±28、214±26和200±24）。COX-2免疫染色评分与T分期显著相关（P<0.05），但与N分期或M分期无关。高分化、中分化和低分化结直肠腺癌的细胞角蛋白20（CK20）阳性表达率分别为97%、94%和65%，这表明CK20可能不是低分化结直肠腺癌与转移性腺癌之间的有效鉴别指标。
