Ruhs Stefanie, Nass Norbert, Somoza Veronika, Friess Ulrich, Schinzel Reinhard, Silber Rolf-Edgar, Simm Andreas
Department of Cardiothoracic Surgery, University of Halle-Wittenberg, Halle, Germany.
Mol Nutr Food Res. 2007 Apr;51(4):488-95. doi: 10.1002/mnfr.200600155.
Advanced glycation end products (AGE) are associated with a wide range of degenerative diseases. The present investigation aimed at analysing the influence of AGE containing nutritional extracts on cardiac fibroblasts (CFs) as the major cell type responsible for cardiac fibrosis. Mice CFs were treated with bread crust extract (BCE) which contained significant amounts of a variety of AGE modifications. BCE treatment with up to 30 mg/mL did not impair cell viability. Furthermore, BCE induced a moderate elevation of reactive oxygen species (ROS) production and activation of redox sensitive pathways like the p42/44(MAPK), p38(MAPK) and NF-kappaB but did not alter Akt kinase phosphorylation. Expression of smooth muscle alpha-actin and tropomyosin-1, which represent markers for myofibroblast differentiation, was reduced after bread crust treatment. These data suggest a putative antifibrotic effect of melanoidin-rich food.
晚期糖基化终末产物（AGE）与多种退行性疾病相关。本研究旨在分析含AGE的营养提取物对心脏成纤维细胞（CFs）的影响，因为CFs是导致心脏纤维化的主要细胞类型。用含有大量多种AGE修饰的面包皮提取物（BCE）处理小鼠CFs。高达30mg/mL的BCE处理不会损害细胞活力。此外，BCE诱导活性氧（ROS）产生适度升高，并激活氧化还原敏感途径，如p42/44（MAPK）、p38（MAPK）和NF-κB，但不会改变Akt激酶磷酸化。面包皮处理后，代表肌成纤维细胞分化标志物的平滑肌α-肌动蛋白和原肌球蛋白-1的表达降低。这些数据表明富含类黑素的食物具有潜在的抗纤维化作用。
