Department of Cardiothoracic Surgery, Martin-Luther University Halle-Wittenberg, Germany.
Exp Gerontol. 2010 Oct;45(10):752-62. doi: 10.1016/j.exger.2010.08.001. Epub 2010 Aug 10.
Advanced glycation end products (AGEs) are considered as biomarkers of ageing and are associated with several degenerative diseases. Besides endogenous formation, significant amounts of AGEs are taken up with food. Although nutritional AGEs are considered as undesirable, proinflammatory agents, they may also enclose potentially beneficial antioxidants. We used rodent cardiac cells to evaluate if food AGEs, present in bread crust, can modify the cellular antioxidant defence. Mice were fed with bread crust containing diet to prove the in-vivo relevance for the heart. In mouse cardiac fibroblasts, bread crust extract induced a moderate elevation of ROS production causing an activation of p42/p44(MAPK), p38(MAPK) and NF-κB, followed by increased expression of antioxidative enzymes. Preconditioning studies demonstrated that this was sufficient to protect cardiac fibroblasts and rat adult cardiac myocytes against severe oxidative stress. Furthermore, mice, fed a bread crust containing diet, exhibited a similarly improved cardiac expression of antioxidative defence genes. The consumption of AGEs can therefore contribute to an improved antioxidant status of the heart, thus exhibiting cardioprotective effects in case of severe oxidative stress as in ischemia reperfusion injury. Also, these data show that the exclusive interpretation of circulating AGEs as pathophysiological biomarkers of ageing might be misleading.
晚期糖基化终产物（AGEs）被认为是衰老的生物标志物，与多种退行性疾病有关。除了内源性形成外，大量的 AGEs 也会随着食物被摄入。尽管营养性 AGEs 被认为是不理想的促炎剂，但它们也可能含有潜在有益的抗氧化剂。我们使用啮齿动物心脏细胞来评估面包皮中存在的食物 AGE 是否可以改变细胞抗氧化防御。用含有面包皮的饮食喂养小鼠以证明其对心脏的体内相关性。在小鼠心肌成纤维细胞中，面包皮提取物诱导 ROS 产生适度升高，导致 p42/p44（MAPK）、p38（MAPK）和 NF-κB 激活，随后抗氧化酶表达增加。预处理研究表明，这足以保护心肌成纤维细胞和大鼠成年心肌细胞免受严重氧化应激。此外，用含有面包皮的饮食喂养的小鼠表现出心脏抗氧化防御基因表达的类似改善。因此，AGE 的消耗可以有助于改善心脏的抗氧化状态，从而在严重氧化应激（如缺血再灌注损伤）情况下表现出心脏保护作用。此外，这些数据表明，将循环 AGEs 单纯解释为衰老的病理生物标志物可能具有误导性。
