Shintani Nahoko, Kurth Tobias, Hunziker Ernst B
ITI Research Institute for Dental and Skeletal Biology, University of Bern, Murtenstrasse 35, 3010 Bern, Switzerland.
J Orthop Res. 2007 Jun;25(6):813-9. doi: 10.1002/jor.20345.
The synovium contains mesenchymal stem cells with chondrogenic potential. Although synovial and articular cartilage tissue develop from a common pool of mesenchymal cells, little is known about their genetic commonalities. In the present study, the mRNA levels for several cartilage-related proteins, namely, cartilage oligomeric matrix protein (COMP), Sox9, aggrecan, and collagen types I, II, IX, X, and XI, were measured using the real-time polymerase chain reaction. Our data reveal the synovium of calf metacarpal joints to physiologically express not only type I collagen but also COMP, Sox9, aggrecan, and collagen types X and XI. The mRNA levels for the latter five proteins lie between 2% and 15% of those in articular cartilage. We speculate that these genes are being expressed by chondroprogenitor cells, whose presence in the synovium reflects a common ontogenetic phase in the fetal development of this tissue and of articular cartilage.
滑膜含有具有软骨形成潜能的间充质干细胞。虽然滑膜和关节软骨组织由共同的间充质细胞池发育而来，但对它们的基因共性了解甚少。在本研究中，使用实时聚合酶链反应测量了几种软骨相关蛋白的mRNA水平，即软骨寡聚基质蛋白（COMP）、Sox9、聚集蛋白聚糖以及I型、II型、IX型、X型和XI型胶原蛋白。我们的数据显示，小牛掌指关节的滑膜不仅在生理上表达I型胶原蛋白，还表达COMP、Sox9、聚集蛋白聚糖以及X型和XI型胶原蛋白。后五种蛋白的mRNA水平介于关节软骨中相应蛋白mRNA水平的2%至15%之间。我们推测这些基因由软骨祖细胞表达，滑膜中软骨祖细胞的存在反映了该组织和关节软骨在胎儿发育过程中的共同个体发生阶段。
