Hsu C-G, Wang P-H, Ko J-L, Chen G-D, Chang H, Yang S-F, Chen S-C, Lin L-Y
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Int J Gynecol Cancer. 2007 Jul-Aug;17(4):851-7. doi: 10.1111/j.1525-1438.2007.00894.x. Epub 2007 Mar 13.
Human telomerase reverse transcriptase (hTERT) and human nonmetastatic clone 23 (nm23-H1) may be separately involved in tumor progression of uterine cervix. We therefore investigate the correlations of hTERT and nm23-H1 in cervical carcinogenesis and further check their application. One hundred and twenty-eight cervical tissues, including 48 squamous cell carcinoma (SCC), 36 high-grade cervical intraepithelial neoplasia (CIN) (CIN 2 and CIN 3), 20 low-grade CIN 1, and 24 normal cases, were collected for immunohistochemical expression of hTERT and nm23-H1. Spearman rank correlation analysis was applied to assess their correlation in these samples. The Fisher exact or Chi-square test was used to evaluate the expression of hTERT or nm23-H1 among each subgroup. The sensitivity, specificity, positive and negative predictive values (PPV and NPV), and accuracy of hTERT and/or nm23-H1 were calculated for the prediction of high-grade CIN and SCC. We found normal cervix and CIN 1 samples had concurrent low expression of hTERT and nm23-H1, whereas high-grade CIN and SCC samples had concurrent high immunoreactivities. The hTERT alone and hTERT or nm23-H1 in combination had better sensitivity, NPV, and accuracy. The nm23-H1 alone as well as hTERT and nm23-H1 in combination had better specificity and PPV. Our results reveal a significantly positive relationship between expression of hTERT and nm23-H1 in normal and neoplastic tissues of uterine cervix. We suggest high expression of hTERT alone and hTERT or nm23-H1 in combination can be offered additional molecular information correlated with high-grade CIN and SCC.
人端粒酶逆转录酶（hTERT）和人非转移性克隆23（nm23-H1）可能分别参与子宫颈肿瘤进展。因此，我们研究了hTERT和nm23-H1在子宫颈癌发生中的相关性，并进一步检查它们的应用。收集了128例子宫颈组织，包括48例鳞状细胞癌（SCC）、36例高级别子宫颈上皮内瘤变（CIN）（CIN 2和CIN 3）、20例低级别CIN 1和24例正常病例，用于hTERT和nm23-H1的免疫组织化学表达检测。采用Spearman等级相关分析评估这些样本中它们的相关性。使用Fisher确切概率法或卡方检验评估各亚组中hTERT或nm23-H1的表达情况。计算hTERT和/或nm23-H1预测高级别CIN和SCC的敏感性、特异性、阳性和阴性预测值（PPV和NPV）以及准确性。我们发现正常子宫颈和CIN 1样本中hTERT和nm23-H1同时低表达，而高级别CIN和SCC样本中同时具有高免疫反应性。单独的hTERT以及hTERT或nm23-H1联合使用具有更好的敏感性、NPV和准确性。单独的nm23-H1以及hTERT和nm23-H1联合使用具有更好的特异性和PPV。我们的结果揭示了子宫颈正常和肿瘤组织中hTERT和nm23-H1表达之间存在显著正相关。我们认为单独的hTERT高表达以及hTERT或nm23-H1联合使用可为与高级别CIN和SCC相关的分子信息提供补充。
