Wang P-H, Chang H, Ko J-L, Lin L-Y
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Int J Gynecol Cancer. 2003 May-Jun;13(3):325-30. doi: 10.1046/j.1525-1438.2003.13180.x.
To evaluate the expression of nm23-H1 protein in normal, precancerous, and cancerous tissues of the uterine cervix and its role in cervical carcinogenesis, metastasis and recurrence, 82 cervical specimens, including 30 squamous cell carcinomas (SCC), 19 high- and 13 low-grade squamous intraepithelial lesions (HSILs and LSILs) and 20 normal samples, were stained using immunohistochemical method with streptavidin-biotin peroxidase immunostaining. The Chi-square and Fisher's exact tests, as well as Chi-square test for trend, were used for comparing nm23-H1 expression among normal, LSIL, HSIL, and cancerous tissues. The correlation of nm23 expression with metastasis or recurrence was analyzed using Fisher's exact test. There were significant differences in levels of nm23-H1 expression between LSIL and HSIL (P = 0.016) and between LSIL and SCC (P = 0.004), but not between HSIL and SCC or normal and LSIL samples. Furthermore, a positive relationship was demonstrated for high nm23-H1 protein expression and degree of malignant transformation (P < 0.05). Among the 30 SCC cases, high nm23-H1 expression did not show significant association with either carcinomatous metastasis or recurrence (P = 0.123, 0.372, respectively). High nm23-H1 expression appears related to critical progression of LSIL to HSIL but not to metastasis or recurrence of SCC.
为评估nm23-H1蛋白在子宫颈正常组织、癌前组织和癌组织中的表达及其在宫颈癌发生、转移和复发中的作用，采用链霉亲和素-生物素过氧化物酶免疫染色的免疫组织化学方法，对82例宫颈标本进行染色，其中包括30例鳞状细胞癌（SCC）、19例高级别和13例低级别鳞状上皮内病变（HSIL和LSIL）以及20例正常样本。采用卡方检验、Fisher精确检验以及趋势卡方检验，比较正常组织、LSIL、HSIL和癌组织中nm23-H1的表达。使用Fisher精确检验分析nm23表达与转移或复发的相关性。LSIL与HSIL之间（P = 0.016）以及LSIL与SCC之间（P = 0.004）的nm23-H1表达水平存在显著差异，但HSIL与SCC之间或正常样本与LSIL之间无显著差异。此外，nm23-H1蛋白高表达与恶性转化程度呈正相关（P < 0.05）。在30例SCC病例中，nm23-H1高表达与癌转移或复发均无显著相关性（分别为P = 0.123、0.372）。nm23-H1高表达似乎与LSIL向HSIL的关键进展有关，但与SCC的转移或复发无关。
