Zhao S, Ma H, Zou S, Chen W, Zhao R
Present address: Yunnan Key Laborary of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming 650201, China.
J Vet Med A Physiol Pathol Clin Med. 2007 Feb;54(1):1-6. doi: 10.1111/j.1439-0442.2007.00898.x.
In order to identify the genes involved in the fatness variability, we studied the expression of several genes implicated in the hepatic lipid metabolism of broiler chickens with different fat deposition patterns during embryonic development. The mRNA expression of acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), malic enzyme (ME) and apolipoprotein B100 (apoB100) genes were determined using reverse transcriptase-polymerase chain reaction (RT-PCR). Samples of livers were collected from Arbor Acres (AA) and Sanhuang (SH) chickens on day 9, 14 and 19 of embryonic development as well as at hatching. This study showed that hepatic triglyceride (TG) level was found to increase suddenly during day 14 of embryonic development, to gradually increase thereafter, and to remain relatively constant at hatching. FAS gene expression in AA and SH broilers occurred prior to hatching and at hatching. The gene was expressed more in the former breed. ACC gene expression was observed beginning at the earlier development stage of days 9. No breed difference was observed in ME and apoB gene expression. This study indicated that the expression of lipogenic enzyme genes of the liver in broiler chickens exhibited scheduling during embryogenesis. The ACC gene started to express earlier than the FAS gene during embryonic development. This suggested that embryonic liver synthesized fatty acid, and breed difference was noticed prior to hatching.
为了鉴定与脂肪沉积变化相关的基因，我们研究了在胚胎发育过程中具有不同脂肪沉积模式的肉鸡肝脏脂质代谢相关的几个基因的表达情况。使用逆转录聚合酶链反应（RT-PCR）测定乙酰辅酶A羧化酶（ACC）、脂肪酸合酶（FAS）、苹果酸酶（ME）和载脂蛋白B100（apoB100）基因的mRNA表达。在胚胎发育的第9天、14天和19天以及孵化时，从艾维茵（AA）鸡和三黄（SH）鸡采集肝脏样本。本研究表明，肝脏甘油三酯（TG）水平在胚胎发育第14天突然升高，此后逐渐升高，并在孵化时保持相对稳定。FAS基因在AA和SH肉鸡孵化前及孵化时均有表达，且在前者品种中表达量更高。ACC基因在第9天的早期发育阶段开始表达。ME和apoB基因表达未观察到品种差异。本研究表明，肉鸡肝脏脂肪生成酶基因的表达在胚胎发生过程中呈现出时间规律。在胚胎发育过程中，ACC基因比FAS基因更早开始表达。这表明胚胎肝脏合成脂肪酸，并且在孵化前就注意到了品种差异。
