Guo Rong-ping, Zhong Chong, Shi Ming, Zhang Chang-qing, Wei Wei, Zhang Ya-qi, Li Jin-qing
State Key Laboratory of Oncology in Southern China, Department of Hepatobiliary Oncology, Cancer Center of Sun Yat-sen University, Guangzhou 510060, China.
Zhonghua Wai Ke Za Zhi. 2006 Dec 1;44(23):1626-30.
To investigate the expression and clinical significance of some apoptosis and angiogenesis factors in hepatocellular carcinoma (HCC).
The expression of p53, Survivin, matrix metalloproteinases (MMP)-2, MMP-9 and vascular endothelial growth factor (VEGF) in 90 specimens of HCC was detected by immunohistochemistry. Correlations of the factors to the recurrence of HCC after hepatectomy were analyzed.
The positive rate of p53, Survivin, MMP-2, MMP-9 and VEGF in HCC tissue was 33.3%, 51.1%, 60.0%, 37.8% and 76.7%, respectively. Of the 5 factors, positive correlation only occurred between the expression of MMP-2 and VEGF, MMP-9 and VEGF. The expression of MMP-2, MMP-9 and VEGF was correlated to the recurrence of HCC. The 1-, 2-, and 3-year tumor-free survival rates were significantly higher in MMP-2 (-) group than in MMP-2 (+) group, and the same results were found with MMP-9 and VEGF. Multivariate analysis revealed that macroscopically disseminated nodules, tumor micrometastasis, serum alpha fetal protein (AFP) level, the expression of MMP-9 and VEGF were independent recurrence risk factors in HCC.
Neither p53 nor Survivin is correlated to the recurrence of HCC; MMPs and VEGF are correlated to the recurrence, and can be used to estimate the risk of postoperative recurrence of HCC.
探讨某些凋亡和血管生成因子在肝细胞癌（HCC）中的表达及其临床意义。
采用免疫组织化学法检测90例HCC标本中p53、Survivin、基质金属蛋白酶（MMP）-2、MMP-9和血管内皮生长因子（VEGF）的表达。分析这些因子与肝癌切除术后复发的相关性。
HCC组织中p53、Survivin、MMP-2、MMP-9和VEGF的阳性率分别为33.3%、51.1%、60.0%、37.8%和76.7%。在这5种因子中，仅MMP-2与VEGF、MMP-9与VEGF的表达呈正相关。MMP-2、MMP-9和VEGF的表达与HCC复发相关。MMP-2（-）组的1年、2年和3年无瘤生存率显著高于MMP-2（+）组，MMP-9和VEGF也有相同结果。多因素分析显示，肉眼可见的播散结节、肿瘤微转移、血清甲胎蛋白（AFP）水平、MMP-9和VEGF的表达是HCC独立的复发危险因素。
p53和Survivin均与HCC复发无关；MMPs和VEGF与复发相关，可用于评估HCC术后复发风险。
