Carrion Ricardo, Patterson Jean L, Johnson Curtis, Gonzales Monica, Moreira Carmen R, Ticer Anysha, Brasky Kathleen, Hubbard Gene B, Moshkoff Dmitry, Zapata Juan, Salvato Maria S, Lukashevich Igor S
Southwest Foundation for Biomedical Research, San Antonio, TX, USA.
Vaccine. 2007 May 16;25(20):4093-102. doi: 10.1016/j.vaccine.2007.02.038. Epub 2007 Feb 27.
Lassa virus (LASV) is responsible for the deaths of thousands of people in West Africa annually. Genetic diversity among LASV strains is the highest among the Arenaviridae and represents a great challenge for vaccine development. Guinea pigs vaccinated with a ML29 reassortant vaccine experienced sterilizing immunity and complete protection when challenged on day 30 either with homologous virus or with the distantly related Nigerian isolate. Simultaneous vaccination-challenge or challenge on day 2 after vaccination also protected 60-100% of the animals against both strains, but without sterilizing immunity. These results indicate that simultaneous replication of ML29 and LASV attenuates the virulence of LASV infection.
拉沙病毒(LASV)每年在西非导致数千人死亡。拉沙病毒毒株之间的基因多样性在沙粒病毒科中是最高的,这对疫苗研发构成了巨大挑战。用ML29重组疫苗接种的豚鼠在第30天受到同源病毒或远缘相关的尼日利亚分离株攻击时,获得了绝育免疫和完全保护。同时进行疫苗接种-攻击或在接种后第2天进行攻击也能使60%-100%的动物抵御这两种毒株,但没有绝育免疫。这些结果表明,ML29和LASV的同时复制会减弱LASV感染的毒力。