Zhao Yangyu, Wen Yan, Polan Mary Lake, Qiao Jie, Chen Bertha H
Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA, USA.
Mol Hum Reprod. 2007 May;13(5):343-9. doi: 10.1093/molehr/gam007. Epub 2007 Mar 14.
We compared latent TGF-ss binding protein-1 (LTBP-1) and fibrillin-1 (FBN-1) expression in leiomyomata and myometrium, correlated with leiomyomata size. We studied in vivo and in vitro effects of ovarian steroids using matched leiomyomata and myometrium samples from both phases of the menstrual cycle. Leiomyomata were divided into small (<or=2 cm), medium (3-5 cm) and large (>or=6 cm) groups. We validated LTBP-1 and FBN-1 expression using QPCR, western blot and immunohistochemistry. LTBP-1 and FBN-1 mRNA and protein expressions were higher in the medium-sized group compared with myometrium in the proliferative phase (P = 0.01; P = 0.01). FBN-1 mRNA expression was higher in the secretory phase (P = 0.01). LTBP-1 mRNA and protein expression was higher in the medium group compared with the small and large groups in the proliferative phase (P = 0.04; P = 0.04). No differences between groups were seen in FBN-1 expression in either phase. 17Beta-estradiol (E2) increased mRNA and protein expression of LTBP-1 and FBN-1 in cultured leiomyoma smooth muscle cells (LSMC) (P < 0.05). No change in FBN-1 and LTBP-1 expression was observed when cells were treated with E2 plus progesterone. Estrogen may be involved in LTBP-1 and FBN-1 expression in leiomyomata. Extracellular matrix metabolism may be different in medium-sized leiomyoma.
我们比较了平滑肌瘤和子宫肌层中潜在转化生长因子β结合蛋白-1（LTBP-1）和原纤蛋白-1（FBN-1）的表达，并将其与平滑肌瘤大小相关联。我们使用来自月经周期两个阶段的匹配平滑肌瘤和子宫肌层样本，研究了卵巢类固醇的体内和体外作用。平滑肌瘤分为小（≤2 cm）、中（3 - 5 cm）和大（≥6 cm）三组。我们通过定量聚合酶链反应（QPCR）、蛋白质免疫印迹和免疫组织化学验证了LTBP-1和FBN-1的表达。与增殖期子宫肌层相比，中-sized组的LTBP-1和FBN-1 mRNA及蛋白表达更高（P = 0.01；P = 0.01）。分泌期FBN-1 mRNA表达更高（P = 0.01）。增殖期时，中-sized组的LTBP-1 mRNA和蛋白表达高于小-sized组和大-sized组（P = 0.04；P = 0.04）。两组在任一阶段的FBN-1表达均无差异。17β-雌二醇（E2）增加了培养的平滑肌瘤平滑肌细胞（LSMC）中LTBP-1和FBN-1的mRNA和蛋白表达（P < 0.05）。当细胞用E2加孕酮处理时，未观察到FBN-1和LTBP-1表达的变化。雌激素可能参与平滑肌瘤中LTBP-1和FBN-1的表达。中-sized平滑肌瘤的细胞外基质代谢可能不同。
