Schrott-Fischer A, Kammen-Jolly K, Scholtz A, Rask-Andersen H, Glueckert R, Eybalin M
Department of Otolaryngology, Medical University of Innsbruck, Innsbruck, Austria.
Acta Otolaryngol. 2007 Jan;127(1):13-9. doi: 10.1080/00016480600652123.
Current neurotransmission models based on animal studies on the mammalian inner ear not always reflect the situation in human. Rodents and primates show significant differences in characteristics of efferent innervation as well as the distribution of neuroactive substances.
Immunohistochemistry demonstrates the mammalian efferent system as neurochemically complex and diverse: several neuroactive substances may co-exist within the same efferent terminal. Using light and electron microscopic immunohistochemistry, this study presents a comparative overview of the distribution patterns of choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, GABA, CGRP, and enkephalins within the peripheral nerve fiber systems of the human inner ear.
Human temporal bones were obtained post mortem and prepared according to a pre-embedding immunohistochemical technique to detect immunoreactivities to ChAT, GABA, CGRP, leu- and met-enkephalins at the electron microscopic level.
Immunoreactivities of all the antigens were present within both the lateral and medial efferent systems of the cochlea, whereas only ChAT, GABA, and CGRP were detected in efferent pathways of the vestibular end organs.
基于对哺乳动物内耳的动物研究得出的当前神经传递模型并不总是能反映人类的情况。啮齿动物和灵长类动物在传出神经支配特征以及神经活性物质的分布方面存在显著差异。
免疫组织化学显示哺乳动物传出系统在神经化学上复杂多样：几种神经活性物质可能共存于同一传出终末内。本研究利用光镜和电镜免疫组织化学，对胆碱乙酰转移酶（ChAT）、乙酰胆碱合成酶、γ-氨基丁酸（GABA）、降钙素基因相关肽（CGRP）和脑啡肽在人内耳周围神经纤维系统中的分布模式进行了比较概述。
死后获取人类颞骨，并根据预包埋免疫组织化学技术进行处理，以在电镜水平检测对ChAT、GABA、CGRP、亮氨酸脑啡肽和甲硫氨酸脑啡肽的免疫反应性。
所有抗原的免疫反应性均存在于耳蜗的外侧和内侧传出系统中，而在前庭终器的传出通路中仅检测到ChAT、GABA和CGRP。
