Trinidad Almudena, Ramírez-Camacho Rafael, García-Berrocal José Ramón, Verdaguer José María, Vicente Javier, Daza Rosa
Department of Otorhinolaryngology, Hospital Universitario Puerta de Hierro, Madrid, Spain.
Acta Otolaryngol. 2007 Feb;127(2):132-7. doi: 10.1080/00016480600749994.
This is a suitable model for the study of different features of middle ear inflammation. This model allows manipulations inside the middle ear while preserving relevant structures such as the tympanic membrane, and provides a useful model for the study of interactions between bacterial infection and eustachian tube dysfunction.
Analysis of early and late histological features in an experimental model of Pseudomonas aeruginosa middle ear inoculation in the rat designed for the study of middle ear procedures.
Thirty Wistar rats were inoculated with Pseudomonas aeruginosa in the tympanic bulla followed by the cauterization of the eustachian tube. Culturing of middle ear effusion was carried out at 7 days follow-up and at sacrifice. Processing of the temporal bones for light microscopy was performed at 7, 14, 30 and 60 days.
Early cultures were positive in most cases, thus proving that middle ear inflammation was due to the presence of inoculated Pseudomona aeruginosa. Mucoperiosteal inflammatory changes similar to those observed in human middle ear infection were seen. Acute inflammatory cell infiltration was seen at 7 and 14 days, gradually decreasing to chronic inflammatory changes with fibroplasia at 60 days. Bone resorption was observed at 7 and 14 days, changing to a bony deposition at 30 and 60 days.
这是一个研究中耳炎症不同特征的合适模型。该模型允许在中耳内部进行操作，同时保留诸如鼓膜等相关结构，并为研究细菌感染与咽鼓管功能障碍之间的相互作用提供了一个有用的模型。
分析为研究中耳手术而设计的大鼠铜绿假单胞菌中耳接种实验模型中的早期和晚期组织学特征。
30只Wistar大鼠在鼓泡中接种铜绿假单胞菌，随后烧灼咽鼓管。在随访7天时和处死时进行中耳积液培养。在第7、14、30和60天对颞骨进行光学显微镜处理。
大多数早期培养结果呈阳性，从而证明中耳炎症是由于接种的铜绿假单胞菌所致。观察到与人类中耳感染中相似的黏骨膜炎症变化。在第7天和第14天可见急性炎性细胞浸润，到第60天逐渐转变为伴有纤维增生的慢性炎症变化。在第7天和第14天观察到骨质吸收，在第30天和第60天转变为骨质沉积。
