Mostafa Badr Eldin, Tawfik Somia, Hefnawi Nadia Galal El, Hassan Mohammed Amir, Ismail Fouad Abbas
Department of Otorhinolaryngology, Ain-Shams Faculty of Medicine, Cairo, Egypt.
Acta Otolaryngol. 2007 Mar;127(3):234-9. doi: 10.1080/00016480600794495.
The addition of deferoxamine to gentamicin seems to confer partial functional and histological protection to the cochlea.
Aminoglycosides are known ototoxic agents. The toxicity occurs via an activation process involving the formation of an iron-gentamicin complex with free radical production. Iron chelation will supposedly limit this toxic effect. This study aimed to determine the possible cochleoprotective role of deferoxamine on the ototoxic effect of gentamicin.
Sixty healthy active guinea pigs, weighing 400-600 g, with an average age of 6 months were used. They were divided into three groups. Group 1 received intramuscular gentamicin 8 mg/kg/day, group 2 received gentamicin 8 mg/kg/day and deferoxamine 150 mg/kg twice daily for 19 days and group 3 served as a control. All animals had a baseline measurement of distortion product oto-acoustic emissions. At the end of 33 days they were submitted to another measurement and then the animals were sacrificed and their cochleas were examined histologically by light and transmission electron microscopy.
In group 1 the mean amplitude post-injection ranged from 5.83 dB at 1001 Hz to 22.33 dB at 6348 Hz. In the deferoxamine + gentamicin group the mean amplitude post-injection ranged from 5.10 dB at 1001 Hz, to 24.45 dB at 6348 Hz. This was statistically significant. At 4004, 5042 and 6348 Hz group 2 showed less histological damage than group 1.
在庆大霉素中加入去铁胺似乎能为耳蜗提供部分功能和组织学保护。
氨基糖苷类是已知的耳毒性药物。其毒性通过一个激活过程发生，该过程涉及铁 - 庆大霉素复合物的形成及自由基产生。铁螯合理应会限制这种毒性作用。本研究旨在确定去铁胺对庆大霉素耳毒性作用可能的耳蜗保护作用。
使用60只健康、活跃的豚鼠，体重400 - 600克，平均年龄6个月。它们被分为三组。第1组每天肌肉注射8毫克/千克庆大霉素，第2组每天肌肉注射8毫克/千克庆大霉素且每天两次注射150毫克/千克去铁胺，共19天，第3组作为对照组。所有动物均进行了畸变产物耳声发射的基线测量。在33天结束时，再次进行测量，然后处死动物，通过光学显微镜和透射电子显微镜对其耳蜗进行组织学检查。
在第1组中，注射后平均振幅在1001赫兹时为5.83分贝，在6348赫兹时为22.33分贝。在去铁胺 + 庆大霉素组中，注射后平均振幅在1001赫兹时为5.10分贝，在6348赫兹时为24.45分贝。这具有统计学意义。在4004、5042和6348赫兹时，第2组的组织学损伤比第1组少。
