Mancini Antonio, Milardi Domenico, Bianchi Antonio, Festa Roberto, Silvestrini Andrea, De Marinis Laura, Pontecorvi Alfredo, Meucci Elisabetta
Institute of Internal Medicine, Operative Unit of Endocrinology, Catholic University of the Sacred Heart, Rome, Italy.
Arch Androl. 2007 Jan-Feb;53(1):37-42. doi: 10.1080/01485010600840756.
To investigate seminal antioxidant systems and their correlation with hormonal pattern in varicocele patients, we studied 33 varicocele (VAR) patients (12 oligozoospermic, 21 normozoospermic) and 34 non-VARs (10 idiopathic oligozoospermic, 24 normozoospermic). Non-enzymatic total antioxidant capacity (TAC) was measured using H(2)O(2)-metamyoglobin, which generates the radical form, spectroscopically detectable, of the chromogen 2,2',-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS): time till appearance (Lag) of ABTS*(-) signifies antioxidant concentration. Lag was significantly longer in VARs than controls suggesting ineffective utilization of antioxidants. A significant direct correlation (r = 0.65, p < 0.01) of Lag with sperm count was observed in non-VARs, while in VARs it was inverted, as well as with hematic FSH levels. A multivariate analysis including FSH, Lag, progressive spermatozoa, oligozoospermia and varicocele indicated a strong inverse correlation between FSH and motility (r(2) = 0.31, p > F = 0.0007), not modified by Lag (r(2) = 0.31, p > F = 0.002). Their inverse correlation with Lag may suggest that higher FSH levels, improving sperm antioxidant efficiency, counterbalance varicocele-induced dyspermia.
为了研究精索静脉曲张患者的精液抗氧化系统及其与激素模式的相关性，我们研究了33例精索静脉曲张（VAR）患者（12例少精子症患者，21例精子正常患者）和34例非精索静脉曲张患者（10例特发性少精子症患者，24例精子正常患者）。使用H(2)O(2)-间肌红蛋白测量非酶促总抗氧化能力（TAC），H(2)O(2)-间肌红蛋白会产生发色团2,2'-偶氮双-3-乙基苯并噻唑啉-6-磺酸盐（ABTS）的可通过光谱检测的自由基形式：ABTS*(-)出现的时间（延迟时间）表示抗氧化剂浓度。精索静脉曲张患者的延迟时间明显长于对照组，表明抗氧化剂利用效率低下。在非精索静脉曲张患者中，观察到延迟时间与精子计数呈显著正相关（r = 0.65，p < 0.01），而在精索静脉曲张患者中则相反，延迟时间与血液卵泡刺激素（FSH）水平也呈负相关。一项包括FSH、延迟时间、进行性精子、少精子症和精索静脉曲张的多变量分析表明，FSH与精子活力之间存在强烈的负相关（r(2) = 0.31，p > F = 0.0007），不受延迟时间影响（r(2) = 0.31，p > F = 0.002）。它们与延迟时间的负相关可能表明，较高的FSH水平提高了精子抗氧化效率，抵消了精索静脉曲张引起的精子运动障碍。
