Verma Neelam, Kumar Kuldeep, Kaur Gurnoor, Anand Sneh
Biosensor Technology Lab, Department of Biotechnology, Punjabi University, Patiala, Punjab, India.
Crit Rev Biotechnol. 2007 Jan-Mar;27(1):45-62. doi: 10.1080/07388550601173926.
This article comprises detailed information about L-asparaginase, encompassing topics such as microbial and plant sources of L-asparaginase, treatment with L-asparaginase, mechanism of action of L-asparaginase, production, purification, properties, expression and characteristics of l-asparaginase along with information about studies on the structure of L-asparaginase. Although L-asparaginase has been reviewed by Savitri and Azmi (2003), our effort has been to include recent and updated information about the enzyme covering new aspects such as structural modification and immobilization of L-asparaginase, recombinant L-asparaginase, resistance to L-asparaginase, methods of assay of L-asparagine and L-asparaginase activity using the biosensor approach, L-asparaginase activity in soil and the factors affecting it. Also, side-effects of L-asparaginase treatment in acute lymphoblastic leukemia (ALL) have been discussed in the current review. L-asparaginase has been and is still one of the most widely studied therapeutic enzymes by researchers and scientists worldwide.
本文包含有关L-天冬酰胺酶的详细信息，涵盖L-天冬酰胺酶的微生物和植物来源、L-天冬酰胺酶治疗、L-天冬酰胺酶的作用机制、生产、纯化、性质、表达和特征等主题，以及关于L-天冬酰胺酶结构研究的信息。尽管Savitri和Azmi（2003年）已对L-天冬酰胺酶进行了综述，但我们的努力旨在纳入有关该酶的最新信息，涵盖新的方面，如L-天冬酰胺酶的结构修饰和固定化、重组L-天冬酰胺酶、对L-天冬酰胺酶的抗性、使用生物传感器方法测定L-天冬酰胺和L-天冬酰胺酶活性的方法、土壤中的L-天冬酰胺酶活性及其影响因素。此外，本综述还讨论了L-天冬酰胺酶治疗急性淋巴细胞白血病（ALL）的副作用。L-天冬酰胺酶一直是且仍然是全球研究人员和科学家研究最广泛的治疗性酶之一。
