Mahmood Tariq, Hyder M Zeeshan, Naqvi S M Saqlan
Department of Biochemistry, University of Arid Agriculture Rawalpindi. Rawalpindi, Pakistan.
DNA Seq. 2007 Feb;18(1):26-32. doi: 10.1080/10425170600986688.
Germin and germin-like proteins (GLPs) are water soluble extracellular proteins reportedly expressed in response to some environmental and developmental signals. Some enzymatic activities have also been associated with germin/GLPs. However, their role in overall metabolism has not been fully understood. Significant insight into their function may also be gained by analysis of their promoter. During this study, about 1107 bp 5'region of OsRGLP2 gene was amplified, cloned and sequenced. The sequence analysis by BLAST showed that this promoter sequence has five common regions (CR1-CR5) of different sizes, which are repeated at 3-6 other locations in 30 kb region in which this gene driven by its promoter is located. Interestingly, all the genes driven by promoter harboring these common regions are GLPs/putative germins. Analysis of these common regions located on OsRGLP2 indicated presence of many elements including those for light responsiveness, dehydration and dark induced senescence, stresses (pathogen and salt), plant growth regulators, pollen specific expression and elements related to seed storage proteins. Analysis of the 30 kb germin/GLP clustered region by GenScan detected each gene to have a putative 40 bp promoter which contains TATA box and Dof factor which turned out to be a part of CR2.
胚萌发素和类胚萌发素蛋白（GLPs）是水溶性细胞外蛋白，据报道它们会响应一些环境和发育信号而表达。一些酶活性也与胚萌发素/GLPs相关。然而，它们在整体代谢中的作用尚未完全了解。通过对其启动子的分析也可能获得对其功能的重要见解。在本研究中，扩增、克隆并测序了OsRGLP2基因约1107 bp的5'区域。通过BLAST进行的序列分析表明，该启动子序列有五个不同大小的共同区域（CR1 - CR5），在由其启动子驱动的该基因所在的30 kb区域中的其他3 - 6个位置重复出现。有趣的是，由含有这些共同区域的启动子驱动的所有基因都是GLPs/假定的胚萌发素。对位于OsRGLP2上的这些共同区域的分析表明存在许多元件，包括光响应、脱水和黑暗诱导衰老、胁迫（病原体和盐）、植物生长调节剂、花粉特异性表达以及与种子储存蛋白相关的元件。通过GenScan对30 kb的胚萌发素/GLP聚类区域进行分析，检测到每个基因都有一个推定的40 bp启动子，其中包含TATA框和Dof因子，结果发现它是CR2的一部分。
