Mahmood Tariq, Tahir Tamkeen, Munir Faiza, Shinwari Zabta Khan
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Comput Biol Chem. 2018 Apr;73:206-212. doi: 10.1016/j.compbiolchem.2018.02.015. Epub 2018 Feb 23.
Germins and germin-like proteins from cupin superfamily contribute resistance to heat denaturation, chemical degradation and against plant pathogens, further functions in plant growth and development. In this study, from three different Oryza sativa accessions KS-282 and Pak 7178 and Pak 7865, OsRGLP2 gene promoter region was amplified, sequenced and analyzed. Sequencing data was evaluated via different computational tools. The regulatory elements were predicted by Consite tool and mapping was done. Many transcription factors binding sites were discovered in OsRGLP2 gene promoter; among these factors, HFH-1 having a significant role in germination was picked for further investigation. To study the interaction between HFH-1 and corresponding regulatory factors, HADDOCK Webserver was used. Graphical models for the interactions of HFH-1 and related regulatory elements were studied by graphic molecular system PyMOL. Mapping of cis-acting regulatory elements in OsRGLP2 gene promoter from three rice accessions showed differences in their position and copy number. Important regulatory elements found in OsRGLP2 promoter region were TATA, CAAT Box, ARR1, GATA, AGAAA, CAAT and DNA-binding One Zinc Finger (Dof) factors, few of them contribute to the regulation of plant defensive system, light responses, developmental and growth activities. Furthermore, during DNA interaction studies, it was found that HFH-1 transcription factor participates in hydrogen bonds formation with thymine and adenine bases.
来自杯状蛋白超家族的萌发素和类萌发素蛋白具有抗热变性、化学降解的能力，并能抵御植物病原体，在植物生长发育中发挥进一步的功能。在本研究中，从三个不同的水稻品种KS-282、Pak 7178和Pak 7865中扩增、测序并分析了OsRGLP2基因启动子区域。通过不同的计算工具对测序数据进行评估。利用Consite工具预测调控元件并进行定位。在OsRGLP2基因启动子中发现了许多转录因子结合位点；在这些因子中，挑选出在萌发中起重要作用的HFH-1进行进一步研究。为了研究HFH-1与相应调控因子之间的相互作用，使用了HADDOCK网络服务器。通过图形分子系统PyMOL研究了HFH-1与相关调控元件相互作用的图形模型。对三个水稻品种OsRGLP2基因启动子中顺式作用调控元件的定位显示，它们在位置和拷贝数上存在差异。在OsRGLP2启动子区域发现的重要调控元件有TATA、CAAT框、ARR1、GATA、AGAAA、CAAT和DNA结合单锌指（Dof）因子，其中一些有助于调节植物防御系统、光反应、发育和生长活动。此外，在DNA相互作用研究中发现，HFH-1转录因子参与与胸腺嘧啶和腺嘌呤碱基形成氢键。
