Mohajer-Maghari Behrokh, Amini-Bavil-Olyaee Samad, Webb Rodney A, Coe Imogen R
Department of Biology, York University, 4700 Keele St, Toronto, Ont., Canada.
DNA Seq. 2007 Feb;18(1):80-3. doi: 10.1080/10425170601060830.
To isolate a full-length alpha-tubulin cDNA from an eucestode, Hymenolepis diminuta, a lambda phage cDNA library was constructed. The alpha-tubulin gene was cloned, sequenced and characterized. The H. diminuta alpha-tubulin consisted of 450 amino acids. This protein contained putative sites for all posttranslational modifications as detyrosination/tyrosination at the carboxyl-terminal of protien, phosphorylation at residues R79 and K336, glycylation/glutamylation at residue G445 and acetylation at residue K40. Comparisons of H. diminuta alpha-tubulin with all full-length alpha-tubulin proteins revealed that H. diminuta alpha-tubulin possesses 10 distinctive residues, which are not found in any other alpha-tubulins. Phylogenetic analysis showed that H. diminuta alpha-tubulin has grouped in a separated branch adjacent eucestode and trematodes branch with 92% bootstrap value (1000 replicates). In conclusion, this is the first report of H. diminuta cDNA library construction, cloning and characterization of H. diminuta alpha-tubulin gene.
为了从微小膜壳绦虫这种真绦虫中分离出全长α-微管蛋白cDNA，构建了一个λ噬菌体cDNA文库。对α-微管蛋白基因进行了克隆、测序和表征。微小膜壳绦虫α-微管蛋白由450个氨基酸组成。该蛋白包含所有翻译后修饰的假定位点，如蛋白质羧基末端的去酪氨酸化/酪氨酸化、R79和K336残基处的磷酸化、G445残基处的糖基化/谷氨酰胺化以及K40残基处的乙酰化。将微小膜壳绦虫α-微管蛋白与所有全长α-微管蛋白进行比较，发现微小膜壳绦虫α-微管蛋白具有10个独特的残基，这些残基在任何其他α-微管蛋白中均未发现。系统发育分析表明，微小膜壳绦虫α-微管蛋白聚集在一个与真绦虫和吸虫分支相邻的单独分支中，自展值为92%（1000次重复）。总之，这是关于微小膜壳绦虫cDNA文库构建、微小膜壳绦虫α-微管蛋白基因克隆和表征的首次报道。
