Temeyer Kevin B, Chen Andrew C
Knipling-Bushland U.S. Livestock Insects Research Laboratory, Agricultural Research Service, US Department of Agriculture, 2700 Fredericksburg Road, Kerrville, TX 78028-9184, USA.
DNA Seq. 2007 Apr;18(2):85-91. doi: 10.1080/10425170601060558.
A 2217-nucleotide cDNA presumptively encoding acetylcholinesterase (AChE) of the horn fly, Haematobia irritans (L.) was sequenced. The open reading frame (ORF) encoded a 91 amino acid secretion signal peptide and a 613 amino acid mature protein with 95% identity and 98% similarity to the AChE of Musca domestica (L.). Structural features characteristic of the M. domestica and Drosophila melanogaster AChEs are conserved in the H. irritans AChE. The M. domestica and D. melanogaster AChEs are target sites for organophosphate inhibition as previously shown (Walsh et al. 2001. Biochem. J. 359: 175-181, Kozaki et al. 2002. Appl. Entomol. Zool. 37: 213-218), suggesting that this H. irritans AChE2 may be the target site for organophosphate.
对一段推测编码角蝇（厩螫蝇）乙酰胆碱酯酶（AChE）的2217个核苷酸的cDNA进行了测序。开放阅读框（ORF）编码一个91个氨基酸的分泌信号肽和一个613个氨基酸的成熟蛋白，该成熟蛋白与家蝇的AChE具有95%的同一性和98%的相似性。家蝇和黑腹果蝇AChE的结构特征在厩螫蝇AChE中是保守的。如先前所示，家蝇和黑腹果蝇的AChE是有机磷酸酯抑制的靶位点（Walsh等人，2001年。《生物化学杂志》359：175 - 181；Kozaki等人，2002年。《应用昆虫学与动物学》37：213 - 218），这表明这种厩螫蝇AChE2可能是有机磷酸酯的靶位点。
