Zhang Jin-Peng, Liu Ting-Song, Zheng Jun, Jin Zheng, Zhu Yun, Guo Jiu-Feng, Wang Guo-Ying
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, People's Republic of China.
DNA Seq. 2007 Apr;18(2):138-44. doi: 10.1080/10425170601060764.
Foxtail millet is a gramineous crop with low water requirement. Cloning of osmotic responses-related genes from foxtail millet is a key step for understanding the mechanism of its tolerance to drought. Here we reported the cloning and characterization of a cDNA (SiOPR1) encoding a putative 12-oxophytodienoic acid reductase 1 from foxtail millet by using RACE methods. Sequence analysis showed that SiOPR1 encoded a polypeptide of 374 amino acids with a predicted molecular mass of 41.9 kDa and pI of 5.14. Multiple alignment result showed that OPR1 protein was very conservative among gramineous crops. RNA gel blot analysis results indicated that SiOPR1 was up-regulated by osmotic stress, and its expression was limited in the roots of foxtail millet. However, SiOPR1 expression was not affected by ABA, NaCl and MeJA treatments both in roots and shoots. Therefore, it is suggested that SiOPR1 gene play an important role in response to drought stress.
谷子是一种需水量低的禾本科作物。从谷子中克隆渗透反应相关基因是了解其耐旱机制的关键步骤。在此，我们报道了通过RACE方法从谷子中克隆并鉴定了一个编码假定的12-氧植物二烯酸还原酶1的cDNA（SiOPR1）。序列分析表明，SiOPR1编码一个由374个氨基酸组成的多肽，预测分子量为41.9 kDa，pI为5.14。多重比对结果表明，OPR1蛋白在禾本科作物中非常保守。RNA凝胶印迹分析结果表明，SiOPR1受渗透胁迫上调，其表达在谷子根中受到限制。然而，SiOPR1的表达在根和地上部分均不受ABA、NaCl和MeJA处理的影响。因此，推测SiOPR1基因在响应干旱胁迫中起重要作用。
