Kuruvilla Leena, T R Santhoshkumar, Kartha Chandrasekharan Cheranellore
Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
Endothelium. 2007 Jan-Feb;14(1):35-43. doi: 10.1080/10623320601177353.
Endocardial endothelial cells (EECs), which form the inner lining of the cavities of the heart, are a distinct cell population whose dysfunction can be critical in pathological conditions of heart. Insights into the role and organization of these cells in pathological states of the heart are limited mainly due to a dearth of experimental models. To date no endocardial endothelial cell line is available. The authors attempted to immortalize porcine ventricular EECs by transfecting the cells with human telomerase reverse transcriptase (hTERT). EECs immortalized by ectopic expression of hTERT exhibit phenotypic and functional characteristics similar to primary EECs. The EE cell line could be useful for the study of mechanisms involved in the interaction of EECs with the underlying myocardium and cardiac interstitium and as useful tools in understanding their role in diseased states of heart.
心内膜内皮细胞（EECs）构成心脏腔室的内膜，是一种独特的细胞群体，其功能障碍在心脏病理状态中可能至关重要。由于缺乏实验模型，目前对这些细胞在心脏病理状态下的作用和组织的了解有限。迄今为止，尚无可用的心内膜内皮细胞系。作者试图通过用人端粒酶逆转录酶（hTERT）转染猪心室EECs使其永生化。通过hTERT异位表达永生化的EECs表现出与原代EECs相似的表型和功能特征。该EE细胞系可用于研究EECs与下层心肌和心脏间质相互作用的机制，并作为理解它们在心脏疾病状态中作用的有用工具。
