Rapid hyperspectral fluorescence lifetime imaging.

作者信息

De Beule Pieter, Owen Dylan M, Manning Hugh B, Talbot Clifford B, Requejo-Isidro Jose, Dunsby Christopher, McGinty James, Benninger Richard K P, Elson Daniel S, Munro Ian, John Lever M, Anand Praveen, Neil Mark A A, French Paul M W

机构信息

Department of Physics, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom.

出版信息

Microsc Res Tech. 2007 May;70(5):481-4. doi: 10.1002/jemt.20434.

DOI:10.1002/jemt.20434

PMID:17366615

Abstract

We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue.

摘要