具有高光谱和空间分辨率的非去扫描并行记录双光子高光谱显微镜。
Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution.
作者信息
Chen Szu-Yu, Lu Chiao-Sheng, Yeh Chia-Hua
机构信息
Department of Optics and Photonics, National Central University, 300, Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.
出版信息
Biomed Opt Express. 2014 Jan 2;5(2):338-47. doi: 10.1364/BOE.5.000338. eCollection 2014 Feb 1.
Abstract
A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.
摘要
构建了一种具有非扫描几何结构和平行记录方案的双光子高光谱显微镜，其具有低驻留时间、高光谱分辨率和高空间分辨率。通过平行记录有效降低了驻留时间以减少光损伤，而非扫描几何结构则显著减少了信号损失和光谱失真。进行了两个实验以表明该系统能够解决串扰问题，并在不对光学厚组织进行染色和切片的情况下对其内在荧光团进行光谱分辨。
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