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Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging.使用单丹磺酰戊烷作为蓝色荧光脂滴标记物，实现多色活细胞成像。
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Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells.荧光显微光谱法作为研究纳米颗粒递送至活癌细胞机制的工具。
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Two-photon excited hemoglobin fluorescence.双光子激发血红蛋白荧光。
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Two-photon spectral imaging with high temporal and spectral resolution.具有高时间和光谱分辨率的双光子光谱成像。
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MEMS scanner enabled real-time depth sensitive hyperspectral imaging of biological tissue.微机电系统（MEMS）扫描仪实现了对生物组织的实时深度敏感高光谱成像。
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A method to unmix multiple fluorophores in microscopy images with minimal a priori information.一种在显微镜图像中以最少先验信息解混多个荧光团的方法。
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具有高光谱和空间分辨率的非去扫描并行记录双光子高光谱显微镜。

Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution.

作者信息

Chen Szu-Yu, Lu Chiao-Sheng, Yeh Chia-Hua

机构信息

Department of Optics and Photonics, National Central University, 300, Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.

出版信息

Biomed Opt Express. 2014 Jan 2;5(2):338-47. doi: 10.1364/BOE.5.000338. eCollection 2014 Feb 1.

DOI:10.1364/BOE.5.000338
PMID:24575331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3920867/
Abstract

A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.

摘要

构建了一种具有非扫描几何结构和平行记录方案的双光子高光谱显微镜，其具有低驻留时间、高光谱分辨率和高空间分辨率。通过平行记录有效降低了驻留时间以减少光损伤，而非扫描几何结构则显著减少了信号损失和光谱失真。进行了两个实验以表明该系统能够解决串扰问题，并在不对光学厚组织进行染色和切片的情况下对其内在荧光团进行光谱分辨。