Owen Dylan M, Auksorius Egidijus, Manning Hugh B, Talbot Clifford B, de Beule Pieter A A, Dunsby Christopher, Neil Mark A A, French Paul M W
Chemical Biology Centre, Photonics Group, Department of Physics, Imperial College London, UK.
Opt Lett. 2007 Dec 1;32(23):3408-10. doi: 10.1364/ol.32.003408.
We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.
我们展示了一种时间选通、光学切片的高光谱荧光寿命成像(FLIM)显微镜,其集成了一个可延伸至紫外波段的可调谐超连续谱激发源。该系统能够在光学切片图像中分辨激发光谱、发射光谱和荧光衰减。