Yu Xin-Ling, He Yong-Kang, Xiong Tie, Zhao Ya-Qin, Shi Meng-Zhi, Zhou Jie, Liu Zong-Chuan, Luo Xin-Song, Fu Xiao, He Hong-Bin, Harn D A, Li Yue-Sheng
Hunan Institute of Parasitic Disease, Yueyang 414000, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Dec;24(6):433-6.
To induce protective effect of co-immunization with S. japonicum triose-phosphate isomerase fused to heat shock protein 70 (SjCTPI-Hsp70) plasmid and interleukin-12 (IL-12) DNA vaccines against Schistosoma japonicum (Chinese strain) infection in water buffalo.
Forty-five 8-10 months-old water buffalo from a nonendemic area were divided into three treatment groups each with fifteen buffalo: experimental group A (SjCTPI-Hsp70+IL-12, 300 microg), experimental group B (SjCTPI+IL-12, 300 microg), and control group C (pVAX+IL-12, 300 microg). All buffalo were immunized with a series of 3 intramuscular injections administered once every four weeks. Twenty-eight days postvaccination, water buffalo were percutaneously challenged with 1000 S. japonicum cercariae. Fecal examinations were conducted two days prior, one day prior, and on perfusion day, and the number of hatching miracidia and eggs per gram feces were recorded. Fifty-six days post-infection, the buffalo were sacrificed and perfused via the descending aorta. The recovered adult worms and eggs in liver tissue were counted.
Groups A and B showed a worm reduction rate of 51.2% and 41.5% (chi2=1.89, P>0.05)), female worm reduction of 48.9% and 44.7% (chi2=0.35,P>0.05), fecal egg reduction of 52.1% and 38.3% (chi2=3.84,P<0.05), a reduction of miracidia-hatching rate by 52.1% and 33.2% (chi2=7.30, P<0.01), and liver egg reduction of 61.5% and 42.0% (chi2=7.61 , P<0.01), respectively.
Co-immunization with SjCTPI-Hsp70 and IL-12 DNA vaccines induces protective immunity against S. japonicum in water buffalo.
诱导日本血吸虫磷酸丙糖异构酶与热休克蛋白70融合基因（SjCTPI-Hsp70）质粒和白细胞介素-12（IL-12）DNA疫苗联合免疫对水牛日本血吸虫（中国株）感染产生保护作用。
将45头来自非疫区的8-10月龄水牛分为3个治疗组，每组15头：实验组A（SjCTPI-Hsp70+IL-12，300微克），实验组B（SjCTPI+IL-12，300微克），对照组C（pVAX+IL-12，300微克）。所有水牛均通过肌肉注射进行3次免疫，每4周注射1次。接种疫苗28天后，用水牛经皮感染1000条日本血吸虫尾蚴。在灌注前2天、1天和灌注当天进行粪便检查，记录每克粪便中孵出的毛蚴和虫卵数量。感染后56天，处死水牛并通过降主动脉进行灌注。计数肝脏组织中回收的成虫和虫卵。
A组和B组的减虫率分别为51.2%和41.5%（χ2=1.89，P>0.05），雌虫减少率分别为48.9%和44.7%（χ2=0.35，P>0.05），粪便虫卵减少率分别为52.1%和38.3%（χ2=3.84，P<0.05），毛蚴孵化率降低率分别为52.1%和33.2%（χ2=7.30，P<0.01），肝脏虫卵减少率分别为61.5%和42.0%（χ2=7.61，P<0.01）。
SjCTPI-Hsp70和IL-12 DNA疫苗联合免疫可诱导水牛对日本血吸虫产生保护性免疫。
