Hu Pingcheng, Xia Da, Cui Hongyan, Zhang Pingfang, He Yongkang, Yu Xinling, Sun Zhenqiu
Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078,China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Aug;37(8):854-9. doi: 10.3969/j.issn.1672-7347.2012.08.017.
To determine the immune-protective effect of Japan Schistosoma (Chinese mainland strain) 23 kD membrane protein-heat shock protein (SjC23-Hsp70) DNA vaccine plus adjuvantinduced interleukin-12 (IL-12) plasmid DNA on Schistosoma japonicum infection in water buffalos.
Forty-five health water buffalos (8-10 months old) in non-endemic area of schistosomiasis were randomly assigned into group A (SjC23-Hsp70+IL-12, 300 μg), group B (SjC23+IL-12, 300 μg) and group C (pVAX+IL-12, 300 μg), 15 in each group. Each buffalo was immuned by shoulder intramuscular injection for 3 times, at an interval of 28 days. Twenty-eight days after the last immunization, each buffalo was infected with 1000 Japan cercariae of Schistosoma. Fecal examinations were conducted 2 days and 1 day before the perfusion, and on the day of perfusion. The number of hatching miracidia and eggs per gram feces was recorded. Fifty-six days after the infection, the buffalos were sacrificed and perfused via the descending aorta. The recovered adult worms and eggs in the liver tissue were counted.
We compared group A and B with group C: the estrogen reduction rate was 45.7% and 26.61%; bug reduction rate was 44.51% and 25.84%; the fecal egg reduction rate was 41.1% and 31.63%; the miracidium reduction rate was 48.11% and 38.07%; and the liver egg reduction rate was 43.39% and 31.95%. The above rates in group A were higher than those in group B (P<0.05).
SjC23-Hsp70 DNA vaccine combined with IL-12 may have a significant immunoprotective effect on buffalos.
探讨日本血吸虫（中国大陆株）23kD膜蛋白-热休克蛋白（SjC23-Hsp70）DNA疫苗联合佐剂诱导的白细胞介素-12（IL-12）质粒DNA对水牛日本血吸虫感染的免疫保护作用。
将45头来自血吸虫病非流行区的健康水牛（8-10月龄）随机分为A组（SjC23-Hsp70+IL-12，300μg）、B组（SjC23+IL-12，300μg）和C组（pVAX+IL-12，300μg），每组15头。每头水牛经肩部肌肉注射免疫3次，间隔28天。末次免疫后28天，每头水牛感染1000条日本血吸虫尾蚴。在灌注前2天、1天及灌注当天进行粪便检查，记录每克粪便中孵出的毛蚴和虫卵数量。感染后56天，处死水牛，经降主动脉灌注。计数肝脏组织中回收的成虫和虫卵数量。
A组和B组与C组比较：减虫率分别为45.7%和26.61%；减卵率分别为44.51%和25.84%；粪便虫卵减少率分别为41.1%和31.63%；毛蚴减少率分别为48.11%和38.07%；肝脏虫卵减少率分别为43.39%和31.95%。A组上述各项指标均高于B组（P<0.05）。
SjC23-Hsp70 DNA疫苗联合IL-12对水牛可能具有显著的免疫保护作用。
