Stübiger Gerald, Belgacem Omar
Institute of Chemical Technologies and Analytics, University of Technology, Getreidemarkt 9/164, A-1060 Vienna, Austria.
Anal Chem. 2007 Apr 15;79(8):3206-13. doi: 10.1021/ac062236c. Epub 2007 Mar 17.
Lipids exhibit a broad range of chemical properties that make their analysis quite demanding. Today, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) represents a versatile tool in the field of lipid analysis, also offering the possibility for molecular structural identification using novel MALDI tandem time-of-flight (TOF/TOF) instrumentation. In this study, we evaluated 2,4,6-trihydroxyacetophenone (THAP) for the analysis of various lipid classes including neutral storage lipids (triacylglycerols), polar membrane lipids (glycerophospho- and sphingolipids), and glycosphingolipids. THAP proved to be a versatile matrix for the routine analysis of various lipids from biological samples ("lipidomics"). A sample preparation methodology was established using selective alkali salt doping for subsequent MS/MS experiments. Sodiated and lithiated molecules provided superior structural information on lipids (i.e., acyl group identification); thus, following this approach, both selective peak detection with high sensitivity and more reliable structural information were obtained simultaneously.
脂质具有广泛的化学性质，这使得对其进行分析颇具难度。如今，基质辅助激光解吸/电离质谱（MALDI-MS）是脂质分析领域一种通用的工具，它还为使用新型MALDI串联飞行时间（TOF/TOF）仪器进行分子结构鉴定提供了可能。在本研究中，我们评估了2,4,6-三羟基苯乙酮（THAP）用于分析各类脂质，包括中性储存脂质（三酰甘油）、极性膜脂质（甘油磷脂和鞘脂）以及糖鞘脂。结果证明，THAP是用于对生物样品中的各类脂质进行常规分析（“脂质组学”）的通用基质。我们建立了一种使用选择性碱金属盐掺杂的样品制备方法，用于后续的MS/MS实验。钠化和锂化分子提供了关于脂质的更优结构信息（即酰基鉴定）；因此，采用这种方法，既能同时获得高灵敏度的选择性峰检测，又能得到更可靠的结构信息。
