Stefanelli Paola, Neri Arianna, Carattoli Alessandra, Mastrantonio Paola
Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita', 00161 Rome, Italy.
Diagn Microbiol Infect Dis. 2007 Jun;58(2):241-4. doi: 10.1016/j.diagmicrobio.2006.11.022. Epub 2007 Mar 26.
In this study, we designed primers and probes for the rpoB gene of Neisseria meningitidis to detect rifampicin-resistant strains in a combined use with primers and probes previously described for penicillin intermediate isolates. The assay was set up in the Light Cycler instrument using the Fluorescence Resonance Energy Transfer platform. The method was applied to N. meningitidis strains and to culture-negative cerebrospinal fluids (CSFs) from patients with meningococcal invasive disease. A spiked CSF was used to determine the sensitivity of the assay.
在本研究中，我们设计了用于脑膜炎奈瑟菌rpoB基因的引物和探针，以便与先前描述的用于检测青霉素中度敏感分离株的引物和探针联合使用，来检测耐利福平菌株。该检测方法在Light Cycler仪器上利用荧光共振能量转移平台进行设置。该方法应用于脑膜炎奈瑟菌菌株以及患有脑膜炎球菌侵袭性疾病患者的培养阴性脑脊液（CSF）样本。使用添加了目标菌的脑脊液来确定该检测方法的灵敏度。
