Pham Hong Nhung, Ohkusu Kiyofumi, Mishima Noriko, Noda Makiko, Monir Shah Mohammad, Sun Xiaosong, Hayashi Masahiro, Ezaki Takayuki
Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.
Diagn Microbiol Infect Dis. 2007 Jun;58(2):153-61. doi: 10.1016/j.diagmicrobio.2006.12.019. Epub 2007 Mar 26.
Phylogenetic relations within the family Enterobacteriaceae were analyzed using partial dnaJ sequences of 165 strains belonging to 93 species from 27 enterobacterial genera. The dnaJ phylogeny was in relative agreement with that constructed by 16S rDNA sequences, but more monophyletic groups were obtained from the dnaJ tree than from the 16S rDNA tree. The degree of divergence of the dnaJ gene was approximately 6 times greater than that of 16S rDNA. Also, the dnaJ gene showed the most discriminatory power in comparison with tuf and atpD genes, facilitating clear differentiation of any 2 enterobacterial species by dnaJ sequence analysis. The application of dnaJ sequences to the identification was confirmed by assigning 72 clinical isolates to the correct enterobacterial species. Our data indicate that analysis of the dnaJ gene sequences can be used as a powerful marker for phylogenetic study and identification at the species level of the family Enterobacteriaceae.
利用来自27个肠杆菌属93个种的165株菌株的部分dnaJ序列，分析了肠杆菌科内的系统发育关系。dnaJ系统发育与16S rDNA序列构建的系统发育相对一致，但从dnaJ树中获得的单系类群比从16S rDNA树中更多。dnaJ基因的分歧程度约为16S rDNA的6倍。此外，与tuf和atpD基因相比，dnaJ基因显示出最大的鉴别能力，通过dnaJ序列分析有助于清晰区分任何两种肠杆菌。通过将72株临床分离株归为正确的肠杆菌种，证实了dnaJ序列在鉴定中的应用。我们的数据表明，dnaJ基因序列分析可作为肠杆菌科物种水平系统发育研究和鉴定的有力标记。
