Wesierska-Gadek J, Penner E, Hitchman E, Kier P, Sauermann G
Institute of Tumorbiology-Cancer Research, First Department of Gastroenterology and Hepatology, Vienna, Austria.
Blood. 1992 Feb 15;79(4):1081-6.
Previously we observed that sera from recipients of allogeneic bone marrow transplants who developed extensive chronic graft-versus-host disease (GVHD) intensively stained the nucleolar region of target cells in indirect immunofluorescence microscopy. To identify the target antigens, immunoblotting experiments were performed using isolated nuclei, isolated nucleoli, and purified nuclear and nucleolar proteins as the antigen source. The nucleolar phosphoproteins B23 and C23 were identified as the main target antigens. Eleven of 19 extensive chronic GVHD sera reacted with these nucleolar phosphoproteins. In addition, four sera recognized histone H1, and two sera recognized the nuclear lamins A and C. Our patients reacting with the nucleolar proteins had symptoms resembling that of scleroderma or Sjögren's syndrome.
此前我们观察到，在间接免疫荧光显微镜检查中，发生广泛慢性移植物抗宿主病（GVHD）的同种异体骨髓移植受者的血清会强烈染色靶细胞的核仁区域。为了鉴定靶抗原，使用分离的细胞核、分离的核仁以及纯化的核蛋白和核仁蛋白作为抗原来源进行了免疫印迹实验。核仁磷蛋白B23和C23被鉴定为主要靶抗原。19份广泛慢性GVHD血清中有11份与这些核仁磷蛋白发生反应。此外，4份血清识别组蛋白H1，2份血清识别核纤层蛋白A和C。与核仁蛋白发生反应的我们的患者具有类似于硬皮病或干燥综合征的症状。
