Ochs R, Lischwe M, O'Leary P, Busch H
Exp Cell Res. 1983 Jun;146(1):139-49. doi: 10.1016/0014-4827(83)90332-4.
Nucleolar phosphoproteins B23 and C23 were simultaneously localized in unsynchronized male rat-kangaroo PtK2 cells during mitosis using a mouse monoclonal antibody against protein B23 and a rabbit antibody against protein C23. The distribution of proteins B23 and C23 during mitosis was compared with the distribution of the silver staining protein. During interphase, proteins B23 and C23 were both localized to the nucleolus. As the nucleolus disappeared in prophase, the distribution of protein B23 became nucleoplasmic, whereas most of protein C23 remained associated with the disappearing nucleolus. Throughout metaphase and anaphase protein B23 was found associated with the chromosomes, whereas protein C23 seemed to disappear. When the nucleolus reformed during telophase, protein C23 appeared first in 'prenucleolar bodies' and then in the nucleolus, whereas protein B23 did not appear in the nucleolus until late telophase or early G1 phase. Silver staining during mitosis closely paralleled the distribution of protein C23, supporting previous conclusions that protein C23 is a silver staining nucleolus organizer region (NOR) protein [19, 20].
使用抗蛋白B23的小鼠单克隆抗体和抗蛋白C23的兔抗体，在有丝分裂过程中，对未同步化的雄性大鼠袋鼠PtK2细胞中的核仁磷蛋白B23和C23进行了同时定位。将蛋白B23和C23在有丝分裂过程中的分布与银染蛋白的分布进行了比较。在间期，蛋白B23和C23都定位于核仁。随着核仁在前期消失，蛋白B23的分布变为核质分布，而大多数蛋白C23仍与消失的核仁相关。在整个中期和后期，发现蛋白B23与染色体相关，而蛋白C23似乎消失了。当核仁在末期重新形成时，蛋白C23首先出现在“前核仁体”中，然后出现在核仁中，而蛋白B23直到末期晚期或G1期早期才出现在核仁中。有丝分裂期间的银染与蛋白C23的分布密切平行，支持了先前的结论，即蛋白C23是一种银染核仁组织区（NOR）蛋白[19,20]。
