Pintacuda Guido, John Michael, Su Xun-Cheng, Otting Gottfried
Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia.
Acc Chem Res. 2007 Mar;40(3):206-12. doi: 10.1021/ar050087z.
The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.
镧系离子的顺磁性为通过核磁共振（NMR）光谱快速测定蛋白质-配体复合物的三维（3D）结构提供了绝佳机会。本文展示了如何利用位点特异性结合的镧系离子诱导的赝接触位移（PCSs）与镧系标记蛋白质的3D结构先验知识相结合，来实现（i）NMR光谱的快速归属，（ii）蛋白质-蛋白质复合物的结构测定，以及（iii）鉴定低分子量化合物与蛋白质复合物中的结合模式。总结了将镧系离子位点特异性掺入蛋白质的策略。
