肥胖与2型糖尿病中的单核细胞趋化蛋白-1。胰岛素敏感性研究。

Monocyte chemoattractant protein-1 in obesity and type 2 diabetes. Insulin sensitivity study.

作者信息

Chacón Matilde R, Fernández-Real José Manuel, Richart Cristóbal, Megía Ana, Gómez José Manuel, Miranda Merce, Caubet Enric, Pastor Rosa, Masdevall Carles, Vilarrasa Núria, Ricard Wifredo, Vendrell Joan

机构信息

Research Department, University Hospital of Tarragona Joan XXIII, Rovira i Virgili University, Pere Virgili Institute, Tarragona, Spain.

出版信息

Obesity (Silver Spring). 2007 Mar;15(3):664-72. doi: 10.1038/oby.2007.578.

DOI:10.1038/oby.2007.578

PMID:17372317

Abstract

OBJECTIVE

Our goal was to test any association between human plasma circulating levels of monocyte chemoattractant protein-1 (cMCP-1) and insulin resistance and to compare monocyte chemoattractant protein-1 (MCP-1) adipose tissue gene expression and cMCP-1 in relation with inflammatory markers.

RESEARCH METHODS AND PROCEDURES

cMCP-1 was measured in n = 116 consecutive control male subjects to whom an insulin sensitivity (S(i)) test was performed. Circulating levels of soluble CD14, soluble tumor necrosis factor receptor type 2 (sTNFR2), soluble interleukin-6 (sIL-6), and adiponectin also were measured. Subcutaneous adipose tissue samples were obtained from n = 107 non-diabetic and type 2 diabetic subjects with different degrees of obesity. Real-time polymerase chain reaction was used to measure gene expression of MCP-1, CD68, tumor necrosis factor-alpha (TNF-alpha), and its receptor TNFR2.

RESULTS

In the S(i) study, no independent effect of cMCP-1 levels on insulin sensitivity was observed. In the expression study, in non-diabetic subjects, MCP-1 mRNA had a positive correlation with BMI (r = 0.407, p = 0.003), TNF-alpha mRNA (r = 0.419, p = 0.002), and TNFR2 mRNA (r = 0.410, p = 0.003). In these subjects, cMCP-1 was found to correlate with waist-to-hip ratio (r = 0.322, p = 0.048). In patients with type 2 diabetes, MCP-1 mRNA was up-regulated compared with non-diabetic subjects. TNF-alpha mRNA was found to independently contribute to MCP-1 mRNA expression. In this group, CD68 mRNA was found to correlate with BMI (r = 0.455, p = 0.001).

DISCUSSION

cMCP-1 is not associated with insulin sensitivity in apparently healthy men. TNF-alpha is the inflammatory cytokine associated with MCP-1 expression in subcutaneous adipose tissue.

摘要

目的

我们的目标是检测人血浆中单核细胞趋化蛋白-1（cMCP-1）循环水平与胰岛素抵抗之间的任何关联，并比较单核细胞趋化蛋白-1（MCP-1）脂肪组织基因表达和cMCP-1与炎症标志物的关系。

研究方法和步骤

对n = 116名连续的对照男性受试者进行胰岛素敏感性（S(i)）测试，并测量其cMCP-1水平。还测量了可溶性CD14、可溶性肿瘤坏死因子受体2型（sTNFR2）、可溶性白细胞介素-6（sIL-6）和脂联素的循环水平。从n = 107名不同程度肥胖的非糖尿病和2型糖尿病受试者中获取皮下脂肪组织样本。采用实时聚合酶链反应测量MCP-1、CD68、肿瘤坏死因子-α（TNF-α）及其受体TNFR2的基因表达。

结果

在S(i)研究中，未观察到cMCP-1水平对胰岛素敏感性有独立影响。在表达研究中，在非糖尿病受试者中，MCP-1 mRNA与体重指数（BMI）呈正相关（r = 0.407，p = 0.003）、与TNF-α mRNA呈正相关（r = 0.419，p = 0.002）以及与TNFR2 mRNA呈正相关（r = 0.410，p = 0.003）。在这些受试者中，发现cMCP-1与腰臀比相关（r = 0.322，p = 0.048）。在2型糖尿病患者中，与非糖尿病受试者相比，MCP-1 mRNA上调。发现TNF-α mRNA独立影响MCP-1 mRNA表达。在该组中，发现CD68 mRNA与BMI相关（r = 0.455，p = 0.001）。