Abhyankar Gauri, Reddy V D
Centre for Plant Molecular Biology, Osmania University, Hyderabad 500 007, India.
Indian J Exp Biol. 2007 Mar;45(3):268-71.
A protocol for rapid multiplication of Adhatoda vasica has been developed through nodal explants from field grown mature plants. The maximum number of shoots, i.e., 7.75 +/- 0.392 differentiated from split nodal halves on MS medium supplemented with BA (10.0 mg/l) during 4 weeks of culture. Maximum number of shoots formed per explant increased to ca. 30 within 6 weeks of subculture on medium containing BA (1.0 mg/l) and Kn (1.0 mg/l). The isolated shoots rooted 90% in MS medium containing IBA (0.1 mg/l) in 2 weeks. The rooted plantlets were successfully transferred to soil in glasshouse and subsequently in field. The plantlets rooted in liquid medium did not survive, but those rooted on solid medium showed more than 75% survival. In vitro raised plants grew successfully ex vitro till flowering.
已通过取自田间成熟植株的茎节外植体开发出一种用于紫花霍香蓟快速繁殖的方案。在添加了BA（10.0毫克/升）的MS培养基上培养4周期间，从劈开的茎节半叶分化出的芽数最多，即7.75±0.392个。在含有BA（1.0毫克/升）和激动素（1.0毫克/升）的培养基上继代培养6周内，每个外植体形成的芽数最多增加到约30个。分离出的芽在含有IBA（0.1毫克/升）的MS培养基中2周内生根率达90%。生根的小植株成功转移到温室土壤中，随后移栽到田间。在液体培养基中生根的小植株未能存活，但在固体培养基中生根的小植株存活率超过75%。离体培养的植株在移栽到体外后成功生长直至开花。
