Kubushiro K, Tsukazaki K, Tanaka J, Takamatsu K, Kiguchi K, Mikami M, Nozawa S, Nagai Y, Iwamori M
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo.
Cancer Res. 1992 Feb 15;52(4):803-9.
The acidic glycosphingolipid composition of human uterine endometrial adenocarcinoma was compared with those of normal uterine endometrium at the proliferative and the secretory phases. Upon chemical composition analysis, no significant transformation-associated change of these glycolipids was observed. However, when cancer cells from the patients with human uterine endometrial adenocarcinoma were transferred to culture, the composition of glycosphingolipids, particularly sulfoglycosphingolipids, was significantly altered after the 70th doubling time. I3SO3-GalCer, which was contained in the original tissues of uterine endometrial adenocarcinomas, disappeared completely from the cultured cells at the 70th doubling time, whereas II3SO3-LacCer and ganglio series sulfoglycosphingolipids, which were originally contained in a trace amount or not present at all in the cancer tissues, became the major components in the total acidic glycosphingolipids in the cultured cells. Also, among cell lines established from several gynecological cancers, which include uterine cervical squamous carcinoma, uterine endometrial adenocarcinoma, ovarian clear cell carcinoma, choriocarcinoma, uterine sarcoma, ovarian sarcoma, and vulvar melanoma, only those cells derived from uterine endometrial adenocarcinoma expressed II3SO3-LacCer and ganglio series sulfoglycosphingolipids and the synthetic activities of these sulfoglycolipids, indicating that uterine endometrial adenocarcinoma cells characteristically lose the sulfotransferase to GalCer and acquire the sulfotransferase to LacCer after being transferred to culture in vitro. Thus, the unique sulfoglycosphingolipids and sulfotransferase are useful markers for the characterization of uterine endometrial adenocarcinoma among human gynecological cancers.
将人子宫内膜腺癌的酸性糖鞘脂组成与正常子宫内膜增殖期和分泌期的酸性糖鞘脂组成进行了比较。经化学成分分析，未观察到这些糖脂有与转化相关的显著变化。然而，当将人子宫内膜腺癌患者的癌细胞进行培养时，在第70次倍增时间后，糖鞘脂的组成，尤其是硫代糖鞘脂的组成发生了显著改变。子宫内膜腺癌原始组织中含有的I3SO3 - GalCer在第70次倍增时间时从培养细胞中完全消失，而原本在癌组织中含量微量或根本不存在的II3SO3 - LacCer和神经节系列硫代糖鞘脂，成为培养细胞中总酸性糖鞘脂的主要成分。此外，在从几种妇科癌症建立的细胞系中，包括子宫颈鳞状癌、子宫内膜腺癌、卵巢透明细胞癌、绒毛膜癌、子宫肉瘤、卵巢肉瘤和外阴黑色素瘤，只有源自子宫内膜腺癌的细胞表达II3SO3 - LacCer和神经节系列硫代糖鞘脂以及这些硫代糖脂的合成活性，这表明子宫内膜腺癌细胞在体外培养后特征性地失去了向GalCer的磺基转移酶，并获得了向LacCer的磺基转移酶。因此，独特的硫代糖鞘脂和磺基转移酶是在人类妇科癌症中表征子宫内膜腺癌的有用标志物。
