Tanaka Kyoko, Mikami Mikio, Aoki Daisuke, Kiguchi Kazushige, Ishiwata Isamu, Iwamori Masao
Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan,
Hum Cell. 2015 Jan;28(1):37-43. doi: 10.1007/s13577-014-0100-4. Epub 2014 Sep 12.
Among negatively charged lipids, sulfoglycolipids are known to be expressed by specific cell populations and to be involved in their functions, including in adhesion with functional proteins, modification of ion channels and induction of cellular differentiation. Accordingly, we determined their amounts in several histologically defined types of ovarian carcinoma tissues. Sulfoglycolipids were determined by TLC-immunostaining with monoclonal anti-sulfatide antibodies and the gene expression of their synthetic enzymes was by RT-PCR. All types of ovarian carcinomas were revealed to exhibit potential to synthesize sulfoglycolipids, either sulfatide (I(3)SO3-GalCer) or sulfated lactosylceramides (II(3)SO3-LacCer), which were expressed at the following frequencies, 6 out of 6 mucinous cystadenocarcinomas, 4 out of 7 serous cystadenocarcinomas, 2 out of 3 endometrioid carcinomas, and 2 out of 3 clear cell adenocarcinomas. All mucinous cystadenocarcinoma tissues preferentially contained sulfatide in amounts of 0.61-1.13 μg per mg dry weight, the molecular species being similar with those of GalCer. Whereas the other carcinomas contained either sulfatide or sulfated LacCer, the latter being detected in 4 out of 6 specimens with sulfoglycolipids. The expression of sulfatide and sulfated LacCer was found to be positively correlated with the amounts of GalCer and LacCer as substrates for sulfotransferase and expression of the genes for GalCer sulfotransferase and ceramide galactosyltransferase. Sulfoglycolipids in ovarian carcinoma tissues were revealed to be expressed in morphologically defined type-characteristic manners, in contrast to the ubiquitous distribution of GM3.
在带负电荷的脂质中，硫酸糖脂已知由特定细胞群体表达并参与其功能，包括与功能蛋白的黏附、离子通道的修饰以及细胞分化的诱导。因此，我们测定了几种组织学定义的卵巢癌组织类型中硫酸糖脂的含量。通过用单克隆抗硫脂抗体进行薄层层析免疫染色来测定硫酸糖脂，并用逆转录聚合酶链反应测定其合成酶的基因表达。结果显示，所有类型的卵巢癌均具有合成硫酸糖脂的潜力，即硫脂（I(3)SO3 - 半乳糖神经酰胺）或硫酸化乳糖基神经酰胺（II(3)SO3 - LacCer），其表达频率如下：6例黏液性囊腺癌中有6例表达，7例浆液性囊腺癌中有4例表达，3例子宫内膜样癌中有2例表达，3例透明细胞腺癌中有2例表达。所有黏液性囊腺癌组织中硫脂含量优先为每毫克干重0.61 - 1.13μg，其分子种类与半乳糖神经酰胺相似。而其他癌组织中要么含有硫脂要么含有硫酸化LacCer，在6例含硫酸糖脂的标本中有4例检测到后者。发现硫脂和硫酸化LacCer的表达与作为硫酸转移酶底物的半乳糖神经酰胺和乳糖基神经酰胺的含量以及半乳糖神经酰胺硫酸转移酶和神经酰胺半乳糖基转移酶基因的表达呈正相关。与GM3的普遍分布不同，卵巢癌组织中的硫酸糖脂以形态学定义的类型特征方式表达。
