Wang Jian Wen, Zheng Li Ping, Tan Ren Xiang
School of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, People's Republic of China.
Appl Microbiol Biotechnol. 2007 Jul;75(5):1183-90. doi: 10.1007/s00253-007-0927-7. Epub 2007 Mar 21.
This work was to characterize the generation of nitric oxide (NO) in Taxus yunnanensis cells induced by a fungal-derived cerebroside and the signal role of NO in the elicitation of plant defense responses and taxol production. (2S,2'R,3R,3'E,4E,8E)-1-O-beta-D-glucopyranosyl-2-N-(2'-hydroxy-3'-octadecenoyl)-3-hydroxy-9-methyl-4,8-sphingadienine at 10 microg/ml induced a rapid and dose-dependent NO production in the Taxus cell culture, reaching a maximum within 5 h of the treatment. The NO donor sodium nitroprusside (SNP) potentiated cerebroside-induced H(2)O(2) production and cell death. Inhibition of nitric oxide synthase activity by phenylene-1,3-bis(ethane-2-isothiourea) dihydrobromide or scavenging NO by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide partially blocked the cerebroside-induced H(2)O(2) production and cell death. Moreover, NO enhanced cerebroside-induced activation of phenylalanine ammonium-lyase and accumulation of taxol in cell cultures. These results are suggestive of a role for NO as a new signal component for activating the cerebroside-induced defense responses and secondary metabolism activities of plant cells.
本研究旨在表征真菌来源的脑苷脂诱导云南红豆杉细胞中一氧化氮（NO）的产生，以及NO在引发植物防御反应和紫杉醇合成中的信号作用。10微克/毫升的（2S,2'R,3R,3'E,4E,8E）-1-O-β-D-吡喃葡萄糖基-2-N-（2'-羟基-3'-十八碳烯酰基）-3-羟基-9-甲基-4,8-鞘氨醇二烯在红豆杉细胞培养物中诱导了快速且剂量依赖性的NO产生，在处理后5小时内达到最大值。NO供体硝普钠（SNP）增强了脑苷脂诱导的H₂O₂产生和细胞死亡。用1,3-双（乙烷-2-异硫脲）苯二氢溴酸盐抑制一氧化氮合酶活性或用2-（4-羧基苯基）-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物清除NO，部分阻断了脑苷脂诱导的H₂O₂产生和细胞死亡。此外，NO增强了脑苷脂诱导的苯丙氨酸解氨酶的激活以及细胞培养物中紫杉醇的积累。这些结果表明，NO作为一种新的信号成分，在激活脑苷脂诱导的植物细胞防御反应和次生代谢活动中发挥作用。
