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内生真菌诱导的红景天苷和酪醇生物合成及其与信号交叉对话的关系，以及红景天中酶基因表达的机制。

Fungal endophyte-induced salidroside and tyrosol biosynthesis combined with signal cross-talk and the mechanism of enzyme gene expression in Rhodiola crenulata.

机构信息

Institute of Applied Chemistry, Shanxi University, Taiyuan, 030006, China.

Institute of Biotechnology, Shanxi University, Taiyuan, 030006, China.

出版信息

Sci Rep. 2017 Oct 2;7(1):12540. doi: 10.1038/s41598-017-12895-2.

DOI:10.1038/s41598-017-12895-2
PMID:28970519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5624951/
Abstract

Endophyte is a factor that affects the physiology and metabolism of plant. However, limited information is available on the mechanism of interaction between endophyte and plant. To investigate the effects of endophytic fungus ZPRs-R11, that is, Trimmatostroma sp., on salidroside and tyrosol accumulations in Rhodiola crenulata, signal transduction, enzyme gene expression, and metabolic pathway were investigated. Results showed that hydrogen peroxide (HO), nitric oxide (NO), and salicylic acid (SA) involved in fungus-induced salidroside and tyrosol accumulations. NO acted as an upstream signal of HO and SA. No up- or down-stream relationship was observed, but mutual coordination existed between HO and SA. Rate-limiting enzyme genes with the maximum expression activities were UDP-glucosyltransferase, tyrosine decarboxylase (TYDC), monoamine oxidase, phenylalanine ammonialyase (PAL), and cinnamic-4-hydroxylase sequentially. Nevertheless, the genes of tyrosine transaminase and pyruvate decarboxylase only indicated slightly higher activities than those in control. Thus, TYDC and PAL branches were the preferential pathways in ZPRs-R11-induced salidroside and tyrosol accumulation. Trimmatostroma sp. was a potential fungus for promoting salidroside and tyrosol accumulations. The present data also provided scientific basis for understanding complex interaction between endophytic fungus and R. crenulata.

摘要

内生真菌是影响植物生理和代谢的一个因素。然而，内生真菌与植物相互作用的机制的信息有限。为了研究内生真菌 ZPRs-R11（即 Trimmatostroma sp.）对红景天中红景天苷和酪醇积累的影响，研究了信号转导、酶基因表达和代谢途径。结果表明，内生真菌诱导红景天苷和酪醇积累涉及过氧化氢（HO）、一氧化氮（NO）和水杨酸（SA）。NO 作为 HO 和 SA 的上游信号。HO 和 SA 之间没有上下游关系，但存在相互协调。表达活性最大的限速酶基因依次为 UDP-葡萄糖基转移酶、酪氨酸脱羧酶（TYDC）、单胺氧化酶、苯丙氨酸氨裂解酶（PAL）和肉桂酸-4-羟化酶。然而，酪氨酸转氨酶和丙酮酸脱羧酶的基因仅表现出比对照稍高的活性。因此，TYDC 和 PAL 分支是 ZPRs-R11 诱导红景天苷和酪醇积累的首选途径。Trimmatostroma sp. 是一种促进红景天苷和酪醇积累的潜在真菌。本研究还为理解内生真菌与红景天之间复杂的相互作用提供了科学依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/337f87e58706/41598_2017_12895_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/813a5a2857f9/41598_2017_12895_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/156520011aca/41598_2017_12895_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/ac0879a1b856/41598_2017_12895_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/337f87e58706/41598_2017_12895_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/813a5a2857f9/41598_2017_12895_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/156520011aca/41598_2017_12895_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/ac0879a1b856/41598_2017_12895_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325f/5624951/337f87e58706/41598_2017_12895_Fig4_HTML.jpg

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