Thody A J, Penny R J, Clark D, Taylor C
J Endocrinol. 1975 Dec;67(3):385-95. doi: 10.1677/joe.0.0670385.
A sensitive and specific radioimmunoassay for alpha-melanocyte-stimulating hormone (alpha-MSH) was developed. Extracts of the neurointermediate lobe of the rat produced displacement curves which were parallel to those obtained with synthetic alpha-MSH. The mean immunoreactive alpha-MSH concentration in neurointermediate lobes from normal adult rats was 2768 +/- 200 (S.E.M.) ng/lobe. This accounted for approximately 78% of the MSH activity of the neurointermediate lobe as measured by bioassay. Much lower levels of immunoreactive alpha-MSH were found in the anterior lobe of the rat. Extracts of rat serum and plasma also contained immunoreactive alpha-MSH and the mean level was found to be 237 +/- 20 pg/ml. This was slightly lower than the level measured in rat plasma by bioassay. Increased levels of alpha-MSH were found in plasma of rats 1 and 3 h after a single injection of trifluoperazine and after 1-5 min of ether anaesthesia. These changes were reflected by decreases in the alpha-MSH content of the neurointermediate lobe.
开发了一种用于α-黑素细胞刺激素（α-MSH）的灵敏且特异的放射免疫测定法。大鼠神经中间叶提取物产生的置换曲线与用合成α-MSH获得的曲线平行。正常成年大鼠神经中间叶中免疫反应性α-MSH的平均浓度为2768±200（标准误）ng/叶。这约占通过生物测定法测得的神经中间叶MSH活性的78%。在大鼠前叶中发现的免疫反应性α-MSH水平要低得多。大鼠血清和血浆提取物中也含有免疫反应性α-MSH，平均水平为237±20 pg/ml。这略低于通过生物测定法在大鼠血浆中测得的水平。在单次注射三氟拉嗪后1小时和3小时以及乙醚麻醉1 - 5分钟后，大鼠血浆中α-MSH水平升高。这些变化反映在神经中间叶α-MSH含量的降低上。
