Hubacek Jaroslav A, Bohuslavova Romana, Skodova Zdena, Pitha Jan, Bobkova Dagmar, Poledne Rudolf
Institute for Clinical and Experimental Medicine, Prague, Czech Republic and Cardiovascular Research Center, Prague, Czech Republic.
Clin Chem Lab Med. 2007;45(3):316-20. doi: 10.1515/CCLM.2007.056.
The relationship between dietary composition and plasma lipids is to some extent genetically determined. It has been found that variants of some genes (e.g., apolipoprotein E and cholesterol 7-alpha hydroxylase) play an important role in changes in plasma lipid levels in response to dietary intervention. We analyzed the effect of variation in the apolipoprotein (APO) APOA1/C3/A4/A5 gene cluster on decreases in plasma cholesterol levels over an 8-year follow-up study.
Men (n=133) from the Czech population, for which dietary composition has markedly changed (red meat 80-->68 kg/person/year, animal fat 16-->9 kg/person/year, fruits and vegetables 133-->150 kg/person/year) were recruited. APOA1 (G-75>A and C83>T), APOC3 (C-482>T and C3238>G), APOA4 (Thr347>Ser and Gln360His) and APOA5 (T-1131>C, Ser19>Trp and Val153>Met) variants were analyzed by PCR and restriction analysis. Lipid levels were analyzed in 1988 and 1996. Dietary information was obtained from the Institute of Agricultural Economy.
In APOA5 Ser19Ser homozygotes (n=105), plasma cholesterol was relatively stable over the years (6.1+/-1.3 and 5.6+/-1.0 mmol/L in 1988 and 1996), but the decrease was much higher in Trp19 carriers (n=27; 6.5+/-1.6 vs. 5.1+/-1.1 mmol/L). This difference in change is significant at p<0.005. Similarly, a better response to dietary changes was detected in carriers of the common APOA4 haplotypes Thr-347Thr/Gln360Gln and Thr347Ser/Gln360Gln (n=102; 6.3+/-1.3 and 5.5+/-1.1 mmol/L in 1988 and 1996, p<0.001). Total cholesterol was relatively stable over time in carriers (n=18) of at least one His360 allele and/or two Ser347 alleles (5.7+/-1.1 and 5.5+/-0.9 mmol/L in 1988 and 1996, n.s.). Other variants analyzed did not influence the change in lipid measurements over time.
APOA4 and APOA5 variants may play an important role in the individual sensitivity of lipid parameters to dietary composition in men.
饮食成分与血浆脂质之间的关系在一定程度上由基因决定。已发现某些基因的变体（如载脂蛋白E和胆固醇7-α羟化酶）在饮食干预后血浆脂质水平变化中起重要作用。我们在一项为期8年的随访研究中分析了载脂蛋白（APO）APOA1/C3/A4/A5基因簇变异对血浆胆固醇水平降低的影响。
招募了来自捷克人群的男性（n = 133），其饮食成分有显著变化（红肉从80降至68千克/人/年，动物脂肪从16降至9千克/人/年，水果和蔬菜从133增至150千克/人/年）。通过PCR和限制性分析检测APOA1（G - 75>A和C83>T）、APOC3（C - 482>T和C3238>G）、APOA4（Thr347>Ser和Gln360His）和APOA5（T - 1131>C、Ser19>Trp和Val153>Met）变体。在1988年和1996年分析脂质水平。饮食信息来自农业经济研究所。
在APOA5 Ser19Ser纯合子（n = 105）中，多年来血浆胆固醇相对稳定（1988年和1996年分别为6.1±1.3和5.6±1.0毫摩尔/升），但在Trp19携带者（n = 27；1988年为6.5±1.6毫摩尔/升，1996年为5.1±1.1毫摩尔/升）中降低幅度更大。这种变化差异在p<0.005时具有统计学意义。同样，在常见APOA4单倍型Thr - 347Thr/Gln360Gln和Thr347Ser/Gln360Gln的携带者（n = 102；1988年和1996年分别为6.3±1.3和5.5±1.1毫摩尔/升，p<0.001）中检测到对饮食变化的更好反应。在至少携带一个His360等位基因和/或两个Ser347等位基因的携带者（n = 18）中，总胆固醇随时间相对稳定（1988年和1996年分别为5.7±1.1和5.5±0.9毫摩尔/升，无统计学差异）。分析的其他变体未影响脂质测量值随时间的变化。
APOA4和APOA5变体可能在男性脂质参数对饮食成分的个体敏感性中起重要作用。
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