Marchitti Satori A, Orlicky David J, Vasiliou Vasilis
Molecular Toxicology & Environmental Health Sciences Program, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, CO, USA.
Biochem Biophys Res Commun. 2007 May 11;356(3):792-8. doi: 10.1016/j.bbrc.2007.03.046. Epub 2007 Mar 15.
Aldehyde dehydrogenases (ALDHs) are critical enzymes in the metabolism of endogenous and exogenous aldehydes. The human genome contains 19 putatively functional ALDH genes; ALDH3B1 belongs to the ALDH3 family. While recent studies have linked the ALDH3B1 locus to schizophrenia, nothing was known, until now, about the properties and significance of the ALDH3B1 protein. The aim of this study was to characterize the ALDH3B1 protein. Human ALDH3B1 was baculovirus-expressed and found to be catalytically active towards medium- and long-chain aliphatic aldehydes and the aromatic aldehyde benzaldehyde. Western blot analyses indicate that ALDH3B1 is highly expressed in kidney and liver and moderately expressed in various brain regions. ALDH3B1-transfected HEK293 cells were significantly protected against cytotoxicity induced by the lipid peroxidation product octanal when compared to vector-transfected cells. This study shows for the first time the functionality, expression and protective role of ALDH3B1 and indicates a potential physiological role of ALDH3B1 against oxidative stress.
醛脱氢酶（ALDHs）是内源性和外源性醛类代谢中的关键酶。人类基因组包含19个推定具有功能的ALDH基因；ALDH3B1属于ALDH3家族。虽然最近的研究已将ALDH3B1基因座与精神分裂症联系起来，但直到现在，关于ALDH3B1蛋白的特性和意义仍一无所知。本研究的目的是对ALDH3B1蛋白进行表征。人ALDH3B1通过杆状病毒表达，发现其对中链和长链脂肪醛以及芳香醛苯甲醛具有催化活性。蛋白质印迹分析表明，ALDH3B1在肾脏和肝脏中高表达，在各个脑区中中等表达。与载体转染的细胞相比，ALDH3B1转染的HEK293细胞对脂质过氧化产物辛醛诱导的细胞毒性具有显著的保护作用。本研究首次展示了ALDH3B1的功能、表达和保护作用，并表明ALDH3B1在抵抗氧化应激方面具有潜在的生理作用。
