School of Biomolecular and Physical Sciences, Griffith University, Nathan, Brisbane, Queensland, Australia.
Chem Biol Interact. 2011 May 30;191(1-3):113-21. doi: 10.1016/j.cbi.2011.01.014. Epub 2011 Feb 4.
Mammalian ALDH3 genes (ALDH3A1, ALDH3A2, ALDH3B1 and ALDH3B2) encode enzymes of peroxidic and fatty aldehyde metabolism. ALDH3A1 also plays a major role in anterior eye tissue UV-filtration. BLAT and BLAST analyses were undertaken of several vertebrate genomes using rat, chicken and zebrafish ALDH3-like amino acid sequences. Predicted vertebrate ALDH3 sequences and structures were highly conserved, including residues involved in catalysis, coenzyme binding and enzyme structure as reported by Liu et al. [27] for rat ALDH3A1. Phylogeny studies of human, rat, opossum, platypus, chicken, xenopus and zebrafish ALDH3-like sequences supported three hypotheses: (1) the mammalian ALDH3A1 gene was generated by a tandem duplication event of an ancestral vertebrate ALDH3A2 gene; (2) multiple mammalian and chicken ALDH3B-like genes were generated by tandem duplication events within genomes of related species; and (3) vertebrate ALDH3A and ALDH3B genes were generated prior to the appearance of bony fish more than 500 million years ago.
哺乳动物 ALDH3 基因（ALDH3A1、ALDH3A2、ALDH3B1 和 ALDH3B2）编码过氧化物和脂肪酸醛代谢的酶。ALDH3A1 在前眼部组织的紫外线过滤中也起着主要作用。使用大鼠、鸡和斑马鱼的 ALDH3 样氨基酸序列对几种脊椎动物基因组进行了 BLAT 和 BLAST 分析。预测的脊椎动物 ALDH3 序列和结构高度保守，包括参与催化、辅酶结合和酶结构的残基，如 Liu 等人 [27] 报道的大鼠 ALDH3A1。人、大鼠、负鼠、鸭嘴兽、鸡、非洲爪蟾和斑马鱼 ALDH3 样序列的系统发育研究支持三个假说：（1）哺乳动物 ALDH3A1 基因是由祖先脊椎动物 ALDH3A2 基因的串联重复事件产生的；（2）多个哺乳动物和鸡的 ALDH3B 样基因是在相关物种基因组内的串联重复事件中产生的；（3）脊椎动物 ALDH3A 和 ALDH3B 基因是在 5 亿多年前骨鱼出现之前产生的。
