Vakoc Benjamin J, Shishko Milen, Yun Seok H, Oh Wang-Yuhl, Suter Melissa J, Desjardins Adrien E, Evans John A, Nishioka Norman S, Tearney Guillermo J, Bouma Brett E
Wellman Center for Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard-MIT Division of Health Sciences and Technology, Boston, Massachusetts, USA.
Gastrointest Endosc. 2007 May;65(6):898-905. doi: 10.1016/j.gie.2006.08.009. Epub 2007 Mar 26.
Optical coherence tomography (OCT) has been used for high-resolution endoscopic imaging and diagnosis of specialized intestinal metaplasia, dysplasia, and intramucosal carcinoma of the esophagus. However, the relatively slow image-acquisition rate of the present OCT systems inhibits wide-field imaging and limits the clinical utility of OCT for diagnostic imaging in patients with Barrett's esophagus.
This study describes a new optical imaging technology, optical frequency-domain imaging (OFDI), derived from OCT, that enables comprehensive imaging of large esophageal segments with microscopic resolution.
A prototype OFDI system was developed for endoscopic imaging. The system was used in combination with a balloon-centering catheter to comprehensively image the distal esophagus in swine.
Volumetric images of the mucosa and portions of the muscularis propria were obtained for 4.5-cm-long segments. Image resolution was 7 microm in depth and 30 microm parallel to the lumen, and provided clear delineation of each mucosal layer. The 3-dimensional data sets were used to create cross-sectional microscopic images, as well as vascular maps of the esophagus. Submucosal vessels and capillaries were visualized by using Doppler-flow processing.
Comprehensive microscopic imaging of the distal esophagus in vivo by using OFDI is feasible. The unique capabilities of this technology for obtaining detailed information of tissue microstructure over large mucosal areas may open up new possibilities for improving the management of patients with Barrett's esophagus.
光学相干断层扫描(OCT)已用于高分辨率内镜成像以及食管特殊肠化生、发育异常和黏膜内癌的诊断。然而,目前OCT系统相对较慢的图像采集速率限制了宽视野成像,并限制了OCT在巴雷特食管患者诊断成像中的临床应用。
本研究描述了一种源自OCT的新型光学成像技术——光学频域成像(OFDI),它能够以微观分辨率对大的食管节段进行全面成像。
开发了一种用于内镜成像的OFDI原型系统。该系统与球囊对中导管联合使用,对猪的食管远端进行全面成像。
获得了4.5厘米长节段的黏膜和部分固有肌层的容积图像。图像分辨率在深度上为7微米,与管腔平行方向为30微米,能够清晰勾勒出各黏膜层。三维数据集用于创建食管的横截面微观图像以及血管图谱。通过多普勒血流处理可显示黏膜下血管和毛细血管。
使用OFDI对食管远端进行体内全面微观成像是可行的。该技术在大的黏膜区域获取组织微观结构详细信息的独特能力,可能为改善巴雷特食管患者的管理开辟新的可能性。