Guzeloglu A, Erdem H, Saribay M K, Thatcher W W, Tekeli T
Department of Obstetrics and Gynaecology, College of Veterinary Medicine, Selcuk University, Selcuklu, Konya 42031, Turkey.
Vet Rec. 2007 Mar 24;160(12):404-6. doi: 10.1136/vr.160.12.404.
Fifty-two 15-month-old Holstein heifers were synchronised with single or double injections of prostaglandin F(2alpha), followed by an injection of gonadotrophin-releasing hormone (gnrh) 48 hours later, and inseminated 12 to 14 hours after the injection of gnrh (day 0). Half of them were then injected twice intramuscularly with 1.1 mg/kg flunixin meglumine 12 hours apart, on the evening of day 15 and the morning of day 16, and the other 26 were not treated. Pregnancy was diagnosed by ultrasound 29 and 65 days after they were inseminated. On day 29, 20 of the treated heifers were pregnant compared with 13 of the control heifers (P<0.05); on day 65, 18 of the treated heifers were still pregnant compared with 12 of the control heifers (P<0.10).
52头15月龄的荷斯坦小母牛，通过单次或两次注射前列腺素F(2α)进行同期发情处理，48小时后注射促性腺激素释放激素（GnRH），并在注射GnRH后12至14小时（第0天）进行人工授精。其中一半小母牛在第15天晚上和第16天早上，间隔12小时，分两次肌肉注射1.1毫克/千克氟尼辛葡甲胺，另一半26头不进行处理。在人工授精后29天和65天通过超声诊断妊娠情况。在第29天，接受处理的小母牛中有20头怀孕，而对照小母牛中有13头怀孕（P<0.05）；在第65天，接受处理的小母牛中有18头仍怀孕，对照小母牛中有12头怀孕（P<0.10）。
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